货号：MAB3610

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Bub1 Antibody, clone 14H5 | MAB3610 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes human Bub1. View All» Immunogen: Recombinant human Bub1. View All» Clone: 14H5 View All» Isotype: IgG1 View All» Background Information: The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells. View All» Species Reactivity:

• Human

• Primate
  View All» Application Notes: Western blot using an ECL detection system. Reacts with the ~150 kDa Bub1 protein. Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA. Immunoprecipitation: 1 μg/mL. Preferred cell lysis buffer is RIPA. Final reaction volume is 200 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested capture agent is Protein G. Optimal working dilutions must be determined by the end user. View All» Presentation: Mouse IgG1 in 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. View All» UniProt Number: O43683 View All» Entrez Gene Number: NM_004336.2 View All» Gene Symbol:
  • BUB1

  • hBUB1

  • BUB1A

  • BUB1L

  • EC 2.7.11.1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunoprecipitation

    • Western Blotting

    • Immunocytochemistry
      View All» Entrez Gene Summary: This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. View All» UniProt Summary: FUNCTION: SwissProt: O43683 # Involved in cell cycle checkpoint enforcement. Can interact and phosphorylate BUB3.SIZE: 1085 amino acids; 122375 Da SUBCELLULAR LOCATION: Nucleus (By similarity). Kinetochore (By similarity). Note=Nuclear in interphase cells. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage (By similarity).TISSUE SPECIFICITY: High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.DOMAIN: SwissProt: O43683 CD1 domain directs kinetochore localization and binding to BUB3.PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.DISEASE:SwissProt: O43683 # Defects in BUB1 are associated with tumor formation.SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. & Contains 1 CD1 domain. & Contains 1 protein kinase domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Bub1 Antibody, clone 14H5 | MAB3610 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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