货号：MAB3576

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Caldesmon Antibody, smooth muscle, clone N5/22 | MAB3576 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Caldesmon, smooth muscle. By Western blot the antibody recognizes a protein of 150-kDa. View All» Epitope: smooth muscle View All» Immunogen: Crude smooth muscle extract from normal human adult uterus. View All» Clone: N5/22 View All» Isotype: IgG1 View All» Background Information: Caldesmon is a protein component of the thin filaments of smooth muscle myofibrils. It is also localized in the stress fibers of fibroblasts. Caldesmon was identified as a Ca2+/Calmodulin-binding protein with molecular weight of 120-150kDa (H-Caldesmon) and 70-80kDa (L-Caldesmon). H-Caldesmon is the primary isoform in smooth muscle while L-Caldesmon is most abundant in non-muscle cells. Caldesmon is capable of binding two calmodulin molecules, one at either end of the protein. Additionally, Caldesmon is an actin, myosin, and tropomyosin-binding protein. Human L-Caldesmon is a protein of 538 amino acids with mobility of 80kDa. In vitro, L-Caldesmon inhibits the actomyosin ATPase in an F-Actin-dependent manner. L-Caldesmon may play an important function in motile processes such as secretion and organelle movement. View All» Species Reactivity:

• Bovine

• Human

• Pig

• Rabbit
  View All» Application Notes: Western blot. Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 7% and/or 4-20% (or similar) gradient gel. Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue. Immunocytochemistry Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG. Optimal working dilutions must be determined by the end user. View All» Control: POSITIVE CONTROL: Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial). View All» Presentation: Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt View All» UniProt Number: Q05682 View All» Entrez Gene Number:
  • NM_004342.5

  • NM_033138.2

  • NM_033139.2

  • NM_033140.2

  • NM_033157.2
    View All» Gene Symbol:
    • CALD1

    • Caldesmon

    • CAD

    • L-CAD

    • CDM

    • MGC21352

    • NAG22

    • H-CAD
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. View All» UniProt Summary: FUNCTION: SwissProt: Q05682 # Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.SIZE: 793 amino acids; 93250 Da SUBCELLULAR LOCATION: Note=On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) (By similarity).TISSUE SPECIFICITY: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.DOMAIN: SwissProt: Q05682 The N-terminal part seems to be a myosin/calmodulin- binding domain, and the C-terminal a tropomyosin/actin/calmodulin- binding domain. These two domains are separated by a central helical region in the smooth-muscle form.PTM: In non-muscle cells, phosphorylation by CDC2 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization (By similarity).SIMILARITY: Belongs to the caldesmon family. View All» Brand Family: Chemicon View All» Product Name: Anti-Caldesmon Antibody, smooth muscle, clone N5/22 | MAB3576 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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