货号：MAB3570

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone N1/5 | MAB3570 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Smooth muscle myosin, heavy chain. By Western blot the antibody recognizes a protein of 200-204 kDa. View All» Epitope: smooth muscle heavy chain, SM1 & SM2 View All» Immunogen: Crude smooth muscle extract from normal human adult uterus. View All» Clone: N1/5 (also referred to as SM-M5) View All» Isotype: IgG1 View All» Species Reactivity:

• Bovine

• Human

• Pig

• Rabbit
  View All» Application Notes: Western blot (see application notes below). Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 5% (see application notes). Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum. The antibody has also been used successfully on methyl-Carnoy fixed tissue. Immunocytochemistry Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG. Optimal working dilutions must be determined by the end user. APPLICATION NOTES FOR MAB3570 WESTERN BLOT To achieve good resolution of myosin heavy chain isoforms with distinct molecular weight (200 - 2004 kDa), the following procedure should be followed: 1). Pyrophoshate extraction buffer for sample preparation (see below); run SDS-PAGE in 5% gel. Important: for better resolution of the MHC bands, use electrophoretic buffer with pH 8.2 (i.e. 0.1 less than standard), and prepare resolving gel (5%) with pH 9.0 (not 8.8 as usual). Also help thorough degasing of the resolving gel mixture (H2O, acrylamide, EDTA, pH 9.0, before (!) adding SDS, TEMED and APS). Run SDS-PAGE longer than after the dye front runs off (use 200 kDa MW markers and let it's 200 kDa band run at least to the middle of 8X8 gel (using a big size gel (not the mini-gel!) will enhance the quality of MHC band resolution). Pyrophosphate extraction buffer: (40mM Na4P2O7x10H2O, 1mM MgCl2, 1mM EGTA (add KOH to dissolve EGTA), PMSF, pH 9.5). To extract acto-myosin from tissues/cells, shake minced tissue or cells in cold extraction buffer 1 hr on ice bath (0oC), centrifuge @10,000g for 10 min at +2-8 oC, take supernatant and mix it 1:1 with standard Laemmli sample buffer, boil, run SDS-PAGE in 5% gel (see above). View All» Control: POSITIVE CONTROL: Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial). View All» Presentation: Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide. View All» Storage Conditions: Maintain at 2-8*C in undiluted aliquots up to 6 months after date of receipt View All» UniProt Number: Q6ZMI0 View All» Entrez Gene Number: NM_152994.2 View All» Gene Symbol:
  • CCDC128

  • MGC111781

  • FLJ16566
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunoprecipitation

    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)
      View All» UniProt Summary: SIZE: 780 amino acids; 88314 Da View All» Brand Family: Chemicon View All» Product Name: Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone N1/5 | MAB3570 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://16ao.com/thread-131219.htm
      © Merck Millipore代理MAB3570 Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone N1/5;store at +2℃ to +8℃，产品报价联系微信jinshanbio