货号：04-1039

品牌：Merck Millipore

规格：

目录价：￥3931.00

市场价格：￥3341.35

会员价格：￥3144.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Ras/p21 Antibody, clone EP1125Y, Rabbit | 04-1039 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody detects H-Ras, N-Ras and K-Ras at and around the N-terminus. View All» Molecular Weight: ~21 kDa View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to residues near the N-terminal region of human Ras. View All» Clone: EP1125Y View All» Isotype: IgG View All» Background Information: Ras, a proto-oncogene, is a small G-protein that has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. H-Ras was first discovered as a transforming product the retrovirus Harvey murine virus and K-Ras of Kirten sarcoma virus. Ras is a heavily studied target of both academic and pharmaceutical research because of its implications in various pathways and diseases as well as being mutated in a large number of human cancers. Ras is most notably the activator of the Erk/MAPK kinase pathway as activator of Raf, as well as an activator of PI3 Kinase (PI3K). In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways including the MAPK pathway through its activation of Raf, but also others as well that include PI3 Kinase and Ral GDS. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Flow Cytometry Analysis: Flow cytometric analysis of permeabilized PC-12 cells using Anti-Ras (1:100 dilution).Immunoprecipitation: A 1:30 dilution from a previous lot was used in IP. View All» Control: C6 cell lysate View All» Quality Assurance: Evaluated by Western Blot on C6 cell lysates.Western Blot Analysis: A 1:500,000 dilution of this antibody was used to detect Ras in C6 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P20936 View All» Entrez Gene Number: NP_002515.1 View All» Gene Symbol:
  • CM-AVM

  • CMAVM

  • DKFZp434N071

  • GAP

  • PKWS

  • RASA

  • RASGAP

  • RasGAP

  • p120GAP

  • p120RASGAP
    View All» Alternate Names:
    • GTPase-activating protein

    • RAS p21 protein activator (GTPase activating protein) 1

    • RAS p21 protein activator 1

    • Ras p21 protein activator

    • triphosphatase-activating protein
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Flow Cytometry

      • Immunoprecipitation
        View All» Entrez Gene Summary: This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). View All» UniProt Summary: FUNCTION: Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.SUBUNIT STRUCTURE: Interacts with SQTSM1. Interacts with SPSB1. Interaction with SPSB1 does not promote degradation. SUBCELLULAR LOCATION: Cytoplasm. PTM: The N-terminus is blocked.INVOLVEMENT IN DISEASE: Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome. Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.SEQUENCE SIMILARITIES: Contains 1 C2 domain.Contains 1 PH domain.Contains 1 Ras-GAP domain.Contains 2 SH2 domains.Contains 1 SH3 domain. View All» Product Name: Anti-Ras/p21, clone EP1125Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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