货号：MAB3510

品牌：Merck Millipore

规格：100UL

目录价：￥5207.00

市场价格：￥4425.95

会员价格：￥4165.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Bromodeoxyuridine Antibody, no acid or proteinase required, clone BU-1 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromodeoxyuridine does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine. MAB3510 does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}. View All» Epitope: no acid or proteinase required View All» Immunogen: Bromodeoxyuridine View All» Clone: BU-1 View All» Isotype: IgG2a View All» Background Information: Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. View All» Species Reactivity: All View All» Application Notes: Immunocytochemistry: 1:5000-1:10,000; acid/alcohol fixed cells and tissues (5 parts glacial acetic acid and 95 parts of 95% ethanol; BU-1 may react with traditional fixations, however it has not been closely examined. PROTOCOL FOR SLIDE-BASED LABELING INDEX ASSAY USING MAB3510 Procedure: 1. Assay performed on mononuclear cells isolated from whole blood or bone marrow using Ficoll™ density gradient. 2. Cytospin slides are prepared using 2.5-5.0 x 104 cells per slide (microscope slides - 1x3 inch, PTFE coated, Cell-Line Associates, Inc. #10-1179). 3. Cells are fixed in 95% Ethanol for 10 minutes at room temperature. 4. Wash slide with PBS containing 0.05% Tween 80. 5. Add 100 mL of MAB3510 diluted appropriately (if PTFE-coated slides are used, the antibody will stay in place over the cells). 6. Incubate for 30 minutes at room temperature. 7. Wash slide with PBS/Tween. 8. Add 100 mL of goat anti-mouse conjugated to Rhodamine (AP124R) appropriately diluted in RPMI containing 10% fetal calf serum. 9. Incubate for 30 minutes at room temperature. 10. Wash slides with PBS/Tween and allow to dry. 11. Place a drop of n-propyl gallate on the cell spot and coverslip. 12. Store slides in the dark at 2-8°C until read. Flow Cytometry: 1:10,000 (95% ethanol fixed cells tested) Optimal working dilutions must be determined by end user. View All» Control: Brdu treated cells View All» Purification Method: Unpurified View All» Presentation: UnPurified mouse tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission. View All» Storage Conditions: Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» Alternate Names: BrdU View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Flow Cytometry

• Immunocytochemistry

• Immunohistochemistry
  View All» Brand Family: Chemicon View All» Product Name: Anti-Bromodeoxyuridine Antibody, no acid or proteinase required, clone BU-1 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Mouse View All»
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  © Merck Millipore代理MAB3510 Anti-BrdU Antibody, clone BU-1;抗-BrdU抗体，克隆BU-1store at -20℃，产品报价联系微信jinshanbio