货号：MAB345

品牌：Merck Millipore

规格：50Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-O4 Antibody, clone 81 | MAB345 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Oligodendrocyte marker O4. Also reacts with certain galactolipids in sperm (see Additional Information library for list). View All» Immunogen: Homogenate of white matter of corpus callosum from bovine brain. View All» Clone: 81 (also referred to in the literature as mAB O4) View All» Isotype: IgM View All» Background Information: Oligodendrocytes and astrocytes are derived from common precursor cells, glioblasts (Sommer, 1981). O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatally (Schachner, 1981) and is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in pro-oligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain. Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments. View All» Species Reactivity:

• Chicken

• Human

• Mouse

• Rat
  View All» Application Notes: Immunohistochemistry: 10-20 μg/mL on unfixed, shock frozen tissue. Immunocytochemistry: 10-20 μg/mL on cells fixed with 4% paraformaldehyde. Note: O4 is a sulfatide, which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation. Optimal working dilutions must be determined by the end user. Immunohistochemistry protocol 1. Prepare sections from unfixed, shock frozen tissue. The sections should be 4-5 μm thick. Place the sections on microscope slides. 2. Wash the slide three times for 5 min. each in PBS at room temperature. 3. Block the non-specific binding sites by incubating the sections in a humid chamber with 5% FCS at room temperature for 30 minutes. 4. Wash the slides as described in step 2. 5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour. 6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained. 7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein* solution and incubate in a humid chamber at 37°C for one hour. 8. Wash the slides as described in step 6. 9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy. *HRP or ABC can also be used. Optimal results can be obtained by titrating the primary and secondary antibodies Immunocytochemistry 1. Fix the preparations with 4% paraformaldehyde (in PBS) at room temperature for 10 minutes. O4 is a sulfatide which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation. 2. Wash the slide three times for 5 min. each in PBS at room temperature. 3. Block the non-specific binding sites by incubating the sections in a human chamber with 5% FCS at room temperature for 30 minutes. 4. Wash the slides as described in step 2. 5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour. 6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained. 7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein solution and incubate in a humid chamber at 37°C for one hour. 8. Wash the slides as described in step 6. 9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy. Note: Do not allow the preparations to dry out during staining. View All» Applications Not Recommended:
  • Immunoprecipitation

  • Western Blotting
    View All» Control: Rat cortical stem cells or day 3 cell cultures of brains from mouse embryos View All» Purification Method: Ammonium sulfate precipitation View All» Presentation: Purified immunoglobulin in 0.05M Potassium phosphate buffer, pH 8.0 with 0.3M NaCl and 0.05% sodium azide. View All» Storage Conditions: Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» Alternate Names: Sulfatide View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry
      View All» Brand Family: Chemicon View All» Product Name: Anti-O4 Antibody, clone 81 | MAB345 View All» Concentration: Varies, see lot specific CoA View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 μg View All» Format: Purified View All» Host: Mouse View All»
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