货号：03-245

品牌：Merck Millipore

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目录价：￥5221.00

市场价格：￥4437.85

会员价格：￥4176.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: RIPAb+™ Hexim 2 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Product Overview: RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.Regulates transcription by acting as a general inhibitor of RNA polymerase II. When HEXIM1 is knocked down, HEXIM2 functionally compensates for its association with P-TEFb. HEXIM2 is up-regulated by HMBA (hexamethylene bisacetamide) at protein level. View All» Specificity: This antibody recognizes Hexim 2 at the N-terminus. View All» Molecular Weight: 42 kDa View All» Epitope: N-terminus View All» Immunogen: KLH-conjugated linear peptide corresponding to human Hexim 2 at and around the N-terminus. View All» Background Information: Regulates transcription by acting as a general inhibitor of RNA polymerase II. Similar to HEXIM1, HEXIM2 forms a complex with p-TEFb and 7SK that prevents p-TEFb from activating transcriptional elongation. When HEXIM1 is knocked down, HEXIM2 functionally compensates for its association with P-TEFb. HEXIM2 is up-regulated by HMBA (hexamethylene bisacetamide) at protein level. View All» Species Reactivity:

• Human

• Chimpanzee
  View All» Species Reactivity Note: Demonstrated to react with human. Predicted to react with chimpanzee based on 100% sequence homology. View All» Application Notes: Immunoprecipitation from RIP lysate:Representative lot data.RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-Hexim 2 antibody (Cat. # CS207314). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: Hexim 2) and HeLa lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Hexim 2 antibody (Cat. # CS207314, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) Arrow indicates Hexim 2 at 42 kDa. (Figure 2).Western Blot Analysis: Representative lot data.HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-Hexim 2 (1:10,000) dilution. Proteins were visualized using a Donkey anti-Rabbit conjugated to HRP using a chemiluminescence detection system. Arrow indicates Hexim 2 (~42 kDa). (Figure 3). View All» Control: Includes negative control normal rabbit IgG antibody and control primers specific for the cDNA of human 7SK snRNA. View All» Quality Assurance: RNA Binding Protein Immunoprecipitation:Representative lot data.RIP Lysate prepared from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG (Cat. # PP64B), or 5 µg of Anti-Hexim 2 antibody (Cat. # CS207314) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).Successful immunoprecipitation of Hexim 2-associated RNA was verified by qPCR using RIP Primers 7SK snRNA, (Cat. # CS204336) (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. View All» Purification Method: Affinity Purfied View All» Presentation: Anti-Hexim 2 (Rabbit Polyclonal), . One vial containing 50 µg of affinity purified polyclonal in buffer containing 0.1 M Tris-glycine, 0.105 M NaCl, pH 7.4, and 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C.Normal Rabbit IgG, . One vial containing 125 μg of rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide. Store at -20°C.RIP Primers, 7SK snRNA.One vial containing 75 μL of 5 μM of each primer specific for the cDNA of 7SK snRNA. Store at -20°C.FOR: TCG GTC AAG GGT ATA CGA GTA GCTREV: CCC TAC GTT CTC CTA CAA ATG GA View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q96MH2 View All» Entrez Gene Number: NP_653209 View All» Gene Symbol:
  • HEXIM2

  • L3
    View All» Alternate Names:
    • Hexamethylene bis-acetamide-inducible protein 2

    • MAQ1 paralog

    • hexamethylene-bis-acetamide-inducible transcript 2

    • hexamthylene bis-acetamide inducible 2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • RNA Binding Protein Immunoprecipitation (RIP)

      • Western Blotting
        View All» UniProt Summary: FUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. SUBUNIT STRUCTURE: Homo-oligomer and heterooligomer with HEXIM1; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in testis. HEXIM1 and HEXIM2 are differentially expressed. INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). DOMAIN: The coiled-coil domain mediates oligomerization.SEQUENCE SIMILARITIES: Belongs to the HEXIM family. View All» Product Name: RIPAb+™ Hexim 2 View All» Concentration: 0.7 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 10 assays View All» Format: Affinity Purified View All» Host: Rabbit View All» Packaging: 10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context). View All»
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