货号：03-207

品牌：Merck Millipore

规格：

目录价：￥4294.00

市场价格：￥3649.90

会员价格：￥3435.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Chloroethyl Ubiquitin (HA-tag) View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 25 µg View All» Background Information: The ubiquitin cascade in its many forms includes a large family of cysteine proteases known as deubiquitinylating enzymes (DUBs). Many members are assigned to this enzyme class by sequence similarity but with little evidence for specific biological activity. HA-tagged ubiquitin molecules derivatised by chemical ligation at the C-terminus with thiol-reactive functional groups can be used as potent, irreversible inhibitors of DUBs through their covalent modification of the active site and, consequently, can act as specific probes for enzymes with DUB activity. Such probes have been utilized for the identification of DUBs and associated components from cell lysates by proteomic methods, as well as rapid demonstration of enzymatic activity for gene products whose functions were inferred from primary structure. The nature of the electrophilic group used as the C-terminal derivative, for example vinyl sulfone (-VS), vinyl methylester (-VME), 2-bromoethyl (-Br) or 2-chloroethyl (-Cl), may determine the subset of DUB enzymes detected. View All» Key Applications: Protein Binding View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Molecular Weight: 10 kDa View All» Brand Family: Upstate View All» Source: Synthetic peptide View All» Presentation: Lyophilized;reconstitute with aqueous buffer up to 5 mg/mL (~500 µM) View All» Storage Conditions: After opening, prepare aliquots and store at -80ºC. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots, store at -80ºC and use within 1 month. Stable for at least 6 months after receipt when stored at -80ºC. View All» Product Name: Chloroethyl Ubiquitin (HA-tag) View All» Physical Form: Frozen solution View All» Format: Purified View All» Purity: >95% by HPLC View All» Purification Method: HPLC View All»