货号：MAB3392

品牌：Merck Millipore

规格：100Ug

目录价：￥4967.00

市场价格：￥4221.95

会员价格：￥3973.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Collagen Type III Antibody, clone IE7-D7 | MAB3392 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody detects collagen type III. There is no evidence for cross reactivity with Collagen Types I, V and VI or connective tissue proteins (Elastin, Fibronectin and Laminin) at suggested working concentrations. View All» Molecular Weight: 138 kDa calculated View All» Epitope: N-terminus View All» Immunogen: Human type III collagen (Werkmeister, J.A., et al. 1990). View All» Clone: 1E7-D7 View All» Isotype: IgG1κ View All» Background Information: Type III collagen (also known as COL3A1), which adds structure and strength to connective tissues, is found in many places in the body, especially skin, lung, intestinal walls, and the walls of blood vessels. Collagen type III is initially produced as pro-collagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the pro-collagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. Other enzymes add sugars to the protein. The type III pro-collagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen's tissue strengthening function. View All» Species Reactivity:

• Rat

• Human
  View All» Species Reactivity Note: Demonstrated to react with rat. Predicted to react with human based on 100% sequence homology. This clone displays a high affinity for human, dog, rat, kangaroo and porcine Type III Collagens. View All» Application Notes: ELISA Analysis: A previous lot of this antibody was used in ELISA (Werkmeister, J.A., et al., 1991).Western Blot Analysis: A previous lot of this antibody was used to detect collagen type III in western blot under non-reduced conditions (Werkmeister J.A., et al., 1988; Ramshaw, J.S., et al., 1988). Some Collagen samples can be contaminated with other Collagen Types. When purified Collagen is used in an application the purity of the Collagen sample should be verified by SDS-page to minimize the risk of false positives.Immunohistochemistry Analysis: A previous lot of this antibody was used to detect collagen type III in immunohistochemistry (Werkmeister J.A., et al., 1989; Werkmeister J.A., et al., 1989; Werkmeister J.A., et al., 1988). View All» Control: Rat knee joint tissue View All» Quality Assurance: Evaluated by Immunohistochemistry in rat knee joint tissue.Immunohistochemistry Analysis: A 1:600 dilution of this antibody detected Collagen Type III in rat knee joint tissue. View All» Purification Method: Protein G Purified View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P02461 View All» Entrez Gene Number: NP_000081 View All» Gene Symbol:
  • EDS4A

  • COL3A1
    View All» Alternate Names:
    • collagen, type III, alpha 1

    • collagen, fetal

    • Ehlers-Danlos syndrome type IV, autosomal dominant

    • alpha1 (III) collagen

    • collagen alpha-1(III) chain
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • ELISA

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]. View All» UniProt Summary: FUNCTION: Collagen type III occurs in most soft connective tissues along with type I collagen.SIZE: 1466 amino acids; 138564 Da SUBUNIT: Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.DISEASE: Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type III (EDS-III) [MIM:130020]; also known as benign hypermobility syndrome. Inheritance is autosomal dominant. EDS is characterized by joint laxity and hyperextensible skin. It is divided into nine different subtypes based on biochemical and clinical variations. & Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type IV (EDS-IV) [MIM:130050]. EDS-IV is the most severe form of the disease, in that it often produces life-threatening consequences, such as rupture of the arteries, bowel, or uterus. A variant form of EDS-IV is Gottron type acrogeria [MIM:201200]. The main characteristics are atrophy and mottled-type hyperpigmentation of the acral skin, resulting in an aged appearance. & Defects in COL3A1 may be a cause of aortic aneurysm [MIM:100070]. Aortic aneurysm consists of a dangerous ballooning of the aorta which is caused by a defect in the artery's wall.SIMILARITY: Belongs to the fibrillar collagen family. & Contains 1 VWFC domain. View All» Product Name: Anti-Collagen Type III, clone IE7-D7 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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