货号：03-105

品牌：Merck Millipore

规格：10assays

目录价：￥6533.00

市场价格：￥5553.05

会员价格：￥5226.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: RIPAb+ Lin28 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.The RIPAb+ Lin28 set includes the Lin28 (Zinc Finger CCHC domain-containing protein 1) antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 68 bp fragment of the human cDNA of CDK4. The Lin28 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of Lin28-associated RNA. View All» Specificity: Lin28 View All» Molecular Weight: 25 kDa View All» Immunogen: The Lin28 antibody is made against full-length His-tagged human Lin28 recombinant protein. View All» Background Information: Lin28 is a developmentally regulated RNA binding protein (RBP). It is down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). If this degradation does not occur, then normal development of various somatic cells fails. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: RNA Binding Protein Immunoprecipitation:RIP Lysate prepared from NTera-2 embryonic carcinoma cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µl of either a normal rabbit IgG, or Anti-Lin28 antibody and the Magna RIP™ Kit (Cat. # 17-700). Successful immunoprecipitation of Lin28-associated RNA was verified by qPCR using primers for U1snRNA as a negative control and positive control RIP Primers CDK4 (Please see figures). Data is presented as percent input of each IP sample relative to input total RNA for each amplicon and RIP sample as indicated. Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.Immunoprecipitation from RIP lysate:RIP lysate from NTera-2 embryonic carcinoma cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or Anti-Lin28 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Lin28 (1.0 µg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures). View All» Control: Included negative control rabbit IgG antibody and control primers specific for CDK4. View All» Quality Assurance: RNA Binding Protein Immunoprecipitation:RIP Lysate prepared from NTera-2 embryonic carcinoma cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG, or Anti-Lin28 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).Successful immunoprecipitation of Lin28-associated RNA was verified by qPCR using RIP Primers CDK4, (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. View All» Presentation: Anti-Lin28 (Rabbit Polyclonal IgG). One vial containing 50 µL of rabbit serum. Store at -20°C.Normal Rabbit IgG. One vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.RIP Primers, CDK4. One vial containing 75 μL of 5 μM of each primer specific for the cDNA of human CDK4. Store at -20°C.FOR: ATG TTG TCC GGC TGA TGG AREV: CAC CAG GGT TAC CTT GAT CTC C View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Store at -80°C for longer term storage. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q9H9Z2 View All» Entrez Gene Number: NP_078950 View All» Gene Symbol:
  • Lin28

  • Lin28A

  • CSDD1

  • ZCCHC1
    View All» Alternate Names:
    • Lin28 homolog A

    • Zinc finger CCHC domain-containing protein 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • RNA Binding Protein Immunoprecipitation (RIP)

      • Immunoprecipitation

      • Western Blotting
        View All» UniProt Summary: FUNCTION: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression By similarity.SUBUNIT STRUCTURE: During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent By similarity.SUBCELLULAR LOCATION: Cytoplasm. Nucleus › nucleolus. Note= Nucleolar localization observed in 10 - 15% of the nuclei in differentiated myotubes By similarity.SPECIFICITY: Expressed in embryonic stem cells (ES cells), placenta and testis.DEVELOPMENTAL STAGE: Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.INDUCTION: Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid.DOMAIN: The CSD domain is required for function in muscle differentiation By similarity.SIMILARITY: Belongs to the lin-28 family.Contains 2 CCHC-type zinc fingers.Contains 1 CSD (cold-shock) domain. View All» Brand Family: Upstate View All» Product Name: RIPAb+ Lin28 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 10 assays View All» Format: Serum View All» Host: Rabbit View All» Packaging: 10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context). View All»
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