货号：03-103

品牌：Merck Millipore

规格：10 assays

目录价：￥5528.00

市场价格：￥4698.80

会员价格：￥4422.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: RIPAb+ SNRNP70 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully coprecipitating a specific RNA target (where such a specific target is known). The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. Each set also includes a negative control antibody to ensure specificity of the RIP reaction.The RIPAb+ SNRNP70 set includes the SNRNP70 antibody, a negative control antibody (purified rabbit IgG), and positive qPCR primers which amplify a 100 bp fragment of the human cDNA of U1snRNA. The SNRNP70 and negative control antibodies are supplied in a scalable "per RIP" reaction size and can be used to functionally validate the precipitation of SNRNP70-associated RNA. View All» Specificity: SNRNP70 View All» Molecular Weight: SNRNP70, observed at ~60 & 48 kDa (Predicted molecular weight ~52 kDa). View All» Immunogen: The SNRNP70 purified antibody is made against a synthetic peptide corresponding to a region of human SNRNP70. View All» Species Reactivity:

• Human

• Canine

• Mouse

• Rat

• Bovine
  View All» Application Notes: Immunoprecipitation from RIP lysate:RIP lysate from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-SNRNP70 antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SNRNP70 (1.0 μg/mL). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) (Please see figures). View All» Control: Included negative control rabbit IgG antibody and control primers specific for U1snRNA. View All» Quality Assurance: RNA Binding Protein Immunoprecipitation:RIP Lysate prepared from HeLa cells (2 X 10⁷ cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or Anti-SNRNP70 antibody and the Magna RIP A Kit (Cat. # 17-700).Successful immunoprecipitation of SNRNP70-associated RNA was verified by qPCR using RIP Primers U1 snRNA (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. View All» Purification Method: Purified View All» Presentation: Anti-SNRNP70 (Rabbit Polyclonal IgG)One vial containing 50 μg of antibody lyophilized from PBS buffer with 2% sucrose. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL. For longer periods of storage store at -20°C. Normal Rabbit IgGOne vial containing 125 μg of purified rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.RIP Primers, U1snRNA One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C.FOR: GGG AGA TAC CAT GAT CAC GAA GGTREV: CCA CAA ATT ATG CAG TCG AGT TTC CC View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P08621 View All» Entrez Gene Number: NP_001009820 View All» Gene Symbol:
  • SNRNP70

  • U1SNRNP

  • RNPU1Z

  • RPU1

  • U1AP1

  • U1SNRNP70

  • SNRPN
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • RNA Binding Protein Immunoprecipitation (RIP)

    • Immunoprecipitation

    • Western Blotting
      View All» UniProt Summary: FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated FUSIP1 and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.SUBCELLULAR LOCATION: Nucleus. POST-TRANSLATIONAL MODIFICATION: The N-terminus is blocked.Extensively phosphorylated on serine residues in the C-terminal region.INVOLVEMENT IN DISEASE: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain. View All» Brand Family: Upstate View All» Product Name: RIPAb+ SNRNP70 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 10 assays View All» Format: Purified View All» Host: Rabbit View All» Packaging: 10 assays per set. Recommended use: ~5 μg antibody per RIP (dependent upon biological context). View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://16ao.com/thread-130971.htm
      © Merck Millipore代理03-103 RIPAb+ Anti-SNRNP70，产品报价联系微信jinshanbio