货号：MAB326

品牌：Merck Millipore

规格：100Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CNPase Antibody, clone 11-5B | MAB326 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc. View All» Molecular Weight: 48 & 46 kDa View All» Immunogen: Purified human brain CNPase View All» Clone: 11-5B View All» Isotype: IgG1 View All» Background Information: CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining. View All» Species Reactivity:

• Bovine

• Canine

• Human

• Mouse

• Pig

• Rat

• Rabbit

• Sheep
  View All» Species Reactivity Note: Bovine, canine, human, mouse, porcine, rat, rabbit and sheep. View All» Non-Reactive Species:
  • Chicken

  • Guinea Pig
    View All» Application Notes: Immunocytochemistry: A previous lot was used on primary oligodendrocyte cultures.Immunohistochemistry: A previous lot was used on rat hippocampus tissue and rat spinal cord.Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development. Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0). Immunoblot: Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sectionsand mixed glial tumors (oligodendrogliomas, etc.)Optimal working dilutions must be determined by end user. View All» Control: Western Blot: Fresh bovine whole brain extract, mouse brain lysate.Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue. View All» Quality Assurance: Routinely evaluated by Western Blot on Mouse Brain lysates.Western Blot Analysis: 1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» UniProt Number: P09543 View All» Entrez Gene Number: NM_033133.4 View All» Gene Symbol:
    • CNP

    • CNPase

    • CNP1

    • EC 3.1.4.37
      View All» Alternate Names: 2'3'-Cyclic Nucleotide 3'-Phosphohydrolase View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)

      • Western Blotting
        View All» UniProt Summary: SIZE: 421 amino acids; 47579 Da SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family. View All» Brand Family: Chemicon View All» Product Name: Anti-CNPase Antibody, clone 11-5B | MAB326 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://16ao.com/thread-130924.htm
        © Merck Millipore代理MAB326 Anti-CNPase Antibody, clone 11-5B;抗磷酸二酯酶抗体，克隆11-5Bstore at +2℃ to +8℃，产品报价联系微信jinshanbio