货号：MAB3118

品牌：Merck Millipore

规格：100UL

目录价：￥7970.00

市场价格：￥6774.50

会员价格：￥6376.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11 | MAB3118 View All» Replaces: AB3250 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: DYKDDDDK sequence containing proteins. Reacts with both N-terminal and C-terminal fusion proteins. View All» Epitope: Epitope tag View All» Immunogen: Synthetic peptide (Cys)-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys coupled to ovalbumin View All» Clone: 2EL-1B11 View All» Isotype: IgG1 View All» Background Information: Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.FLAG® is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. View All» Species Reactivity:

• Vertebrates

• E. coli
  View All» Species Reactivity Note: Expected to react with rat based on sequence homology. View All» Application Notes: Immunofluorescence: A 1:1,000-1:5,000 dilution of a previous lot was used in IF.Immunoprecipitation: A 1:1,000-1:5,000 dilution of a previous lot was used IP on recombinant proteins.ELISA: A 1:1,000-1:5,000 dilution of a previous lot was used in Enzyme-linked immunosorbent assay on recombinant proteins. Note: Does not require Ca++ for binding.Immunoblotting: on recombinant proteinsOptimal working dilutions must be determined by the end user. View All» Control: Cells transfected with FLAG-tagged fusion vector View All» Quality Assurance: Routinely evaluated by Western Blot on Flag-mTor protein blot.Western Blot Analysis: 1:500 dilution of this lot detected Flag mTor on Flag-mTOR blot. View All» Purification Method: Unpurified View All» Presentation: Ascites mouse monoclonal IgG1 liquid containing no preservative. View All» Storage Conditions: Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Immunocytochemistry

  • Immunofluorescence

  • Immunoprecipitation

  • Western Blotting
    View All» Brand Family: Chemicon View All» Product Name: Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11 | MAB3118 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Ascites View All» Host: Mouse View All»
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    © Merck Millipore代理MAB3118 Anti-DYKDDDDK (FLAG® epitope tag) Antibody, clone 2EL-1B11;抗DYKDDDDK（FLAG®表位标签）抗体，克隆2EL-1B11store at，产品报价联系微信jinshanbio