货号：MAB2221

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae View All» Replaces: CBL1324 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Y-Ae is specific to mouse MHC Class II IAb molecule containing the 52-68 peptide of the alpha chain of I-E peptide. This antibody is capable labelling antigen presentation because it specifically binds to the binding site when peptide is present. View All» Epitope: contains Ealpha peptide 52-68, no azide View All» Immunogen: 5R - LPS B cell blasts View All» Clone: Y-Ae View All» Isotype: IgG2bκ View All» Background Information: MHC class I and class II molecules transport foreign and self peptides to the cell surface and present them to T lymphocytes. Detection of these peptide-MHC complexes has thus far been limited to analysis of the response of a T cell. mAb Y-Ae reacts with 10 to 15% of class II molecules on peripheral B lymphocytes and on cells in the thymus medulla, but not thymus cortex in mice, that express both I-Ab and I-Eb molecules. Y-Ae also detects a self E alpha peptide bound to I-Ab molecules. It does not recognize I-ab alone. It does not bind to the peptide groove and block antigen presentation and thus it is a useful tool in tracking antigen presentation because it only binds when the correct peptide + MHC is present. In addition to B lymphocytes, the Y-Ae determinant is expressed at comparable levels on other antigen-presenting cells, including macrophages and dendritic cells. Finally, the antibody does not react with invariant chain-associated class II complexes, thus providing direct evidence that invariant chain-class II complexes and peptide-class II complexes are mutually exclusive. View All» Species Reactivity: Mouse View All» Application Notes: Western Blot Immunohistochemistry: 1-2 μg/mL on frozen tissue with acetone fixation Immunoprecipitation Flow Cytometry: 1-2 μg per 10E6 cells in 100μL volume Optimal working dilutions must be determined by the end user. View All» Presentation: Liquid in 0.2M phosphate, 0.25M NaCl, pH 7.6, containing no preservatives. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P42858 View All» Entrez Gene Number: NM_002111.6 View All» Gene Symbol:

• HTT

• IT15

• SLC6A4

• Huntingtin

• OCD1

• HD
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Flow Cytometry

  • Immunoprecipitation

  • Western Blotting

  • Immunohistochemistry
    View All» Entrez Gene Summary: Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. View All» UniProt Summary: FUNCTION: SwissProt: P42858 # May play a role in microtubule-mediated transport or vesicle function.SIZE: 3144 amino acids; 347860 Da SUBUNIT: Binds SH3GLB1 (By similarity). Interacts through its N- terminus with FNBP3. Interacts with PQBP1, SETD2 and SYVN.SUBCELLULAR LOCATION: Cytoplasm. Nucleus.TISSUE SPECIFICITY: Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.PTM: Cleaved by apopain downstream of the polyglutamine stretch. The resulting amino-terminal fragment is cytotoxic and provokes apoptosis. & Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.DISEASE: SwissProt: P42858 # Defects in HD are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).SIMILARITY: SwissProt: P42858 ## Belongs to the hungtintin family. & Contains 10 HEAT repeats. View All» Brand Family: Chemicon View All» Product Name: Anti-MHC Class II Antibody, contains Eα peptide 52-68, no azide, clone Y-Ae View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 500 µg View All» Format: Purified View All» Host: Mouse View All»
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