货号：MAB2079Z

品牌：Merck Millipore

规格：100Ug

目录价：￥5820.00

市场价格：￥4947.00

会员价格：￥4656.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free | MAB2079Z View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Monoclonal antibody MAB2079Z is specific for active conformation of human beta1 integrins. The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit nonconvalently associated with the common beta1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies such as MAB2079Z which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. Antibody MAB2079Z recognizes epitopes in the 355-425 region of the common beta1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli. View All» Molecular Weight: 88 kDa View All» Epitope: activated, azide free View All» Immunogen: Generated against human beta1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. MAB2079Z was specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation. View All» Clone: HUTS-4 View All» Isotype: IgG2b View All» Background Information: Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. View All» Species Reactivity:

• Human

• Rat
  View All» Application Notes: ELISA Immunohistochemistry: fresh frozen, or light fixation, with 4% PFA permeabilized with 0.1% triton X-100 in the presence of Mn++ as the epitope of HUTS-4 is dependent upon Mn++ ion. Note antibody only stains activated beta1 integrins. Activated beta-1 integrins are typically found in wound regions for instance (see http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=eurekah.figgrp.12951) . Use MAB1981 or CBL1333 for general beta1 integrin staining and detection. Western Blotting: non-reducing conditions only (see: http://www.jbc.org/cgi/content/full/271/19/11067).FACS analysis: 2.5 μg antibody / 5 x 10⁵ cells. View All» Control: Human lung, liver, and skeletal muscle tissues View All» Purification Method: Protein A purfied View All» Presentation: Protein A Purified mouse immunoglobulin in PBS containing no preservatives. View All» Storage Conditions: Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: P05556 View All» Entrez Gene Number: NM_002292.3 View All» Gene Symbol:
  • LAMB2

  • S-laminin

  • LAMS
    View All» Alternate Names:
    • Ligand-inducedBindingSite

    • LIBS

    • CLIBS

    • MAB2079
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Flow Cytometry

      • Immunohistochemistry

      • Western Blotting
        View All» UniProt Summary: FUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.SIZE: 798 amino acids; 88465 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Integrin β1 Antibody, activated, clone HUTS-4, Azide Free | MAB2079Z View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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