货号：07-2270

品牌：Merck Millipore

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目录价：￥3821.00

市场价格：￥3247.85

会员价格：￥3056.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-WNK4 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes WNK4 at the C-terminus. View All» Molecular Weight: ~160 kDa observed. This protein has 3 isoforms produced by alternative splicing. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057. View All» Epitope: C-terminus View All» Immunogen: KLH-conjugated linear peptide corresponding to human WNK4 at the C-terminus. View All» Background Information: WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. Predicted to react with rat based on 100% sequence homology. View All» Application Notes: Immunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected WNK4 in normal human kidney tissue. View All» Control: Mouse kidney cell lysate View All» Quality Assurance: Evaluated by Western Blot in mouse kidney cell lysate.Western Blot Analysis: 0.05 µg/mL of this antibody detected WNK4 on 10 µg of mouse kidney cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q96J92 View All» Entrez Gene Number: NP_115763 View All» Gene Symbol:
  • WNK4

  • PRKWNK4
    View All» Alternate Names:
    • Serine/threonine-protein kinase WNK4

    • Protein kinase lysine-deficient 4

    • Protein kinase with no lysine 4
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB. View All» UniProt Summary: FUNCTION: Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K+ channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K+ secretion to maintain integrated homeostasis (By similarity). CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium (By similarity). ENZYME REGULATION: Activation requires autophosphorylation of Ser-335. Phosphorylation of Ser-331 also promotes increased activity (By similarity). SUBUNIT STRUCTURE: Interacts with the C-terminal region of KCNJ1 (By similarity).SUBCELLULAR LOCATION: Cell junction › tight junction By similarity. Note: Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity).TISSUE SPECIFICITY: Expressed in kidney, colon and skin. INVOLVEMENT IN DISEASE: Defects in WNK4 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.Contains 1 protein kinase domain.CAUTION: Cys-203 is present instead of the conserved Lys which is expected to be an active site residue. Lys-186 appears to fulfill the required catalytic function. UniProtKB Q9JIH7SEQUENCE CAUTION: The sequence BAC04669.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC48387.1 differs from that shown. Reason: Frameshift at position 4. View All» Product Name: Anti-WNK4 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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