货号：MAB1997

品牌：Merck Millipore

规格：100Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Integrin β1 Antibody, clone MB1.2 | MAB1997 View All» Replaces: CBL1333F View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with β1 subunit of VLA (β1) integrins View All» Molecular Weight: 88 kDa; aound 130 kDa with glycosylation View All» Clone: MB1.2 View All» Isotype: IgG2κ View All» Background Information: Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. View All» Species Reactivity:

• Mouse

• Rat
  View All» Species Reactivity Note: Mouse. Expected to react with rat based on sequence homology. View All» Application Notes: Immunoprecipitation: A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.Flow cytometry: A 10 μg/mL concentration of a previous lot was used in FC.Immunohistochemistry in frozen tissue sections: 20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized. Western blot: 10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples. Optimal working dilutions must be determined by end user. View All» Control:
  • Human OVCAR5 cell line whole cell lysate

  • C2C12 lysate.
    View All» Quality Assurance: Routinely evaluated by Western Blot on C2C12 lysate.Western Blot Analysis: 1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate. View All» Purification Method: Protein A purfied View All» Presentation: Purified rat monoclonal IgG2κ liquid in buffer containing 0.02M phosphate buffer, pH 7.6, 0.25M NaCl with 0.1% NaN3 View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» UniProt Number: P05556 View All» Entrez Gene Number: NP_002202 View All» Gene Symbol:
    • ITGB1

    • GPIIA

    • OTTHUMP00000063731

    • OTTHUMP00000046253

    • OTTHUMP00000063733

    • VLAB

    • OTTHUMP00000063732

    • OTTHUMP00000046253

    • MDF2

    • FNRB

    • CD29

    • MSK12

    • VLA-BETA
      View All» Alternate Names:
      • CD29 antigen

      • Fibronectin receptor subunit beta

      • Integrin VLA-4 subunit beta

      • fibronectin receptor beta subunit

      • integrin VLA-4 beta subunit

      • integrin beta 1

      • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
        View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
        • Flow Cytometry

        • Immunohistochemistry

        • Immunoprecipitation

        • Western Blotting
          View All» Entrez Gene Summary: Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. SUBUNIT STUCTURE: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha-5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5. Interacts with FLNA, FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with KRT1 in the presence of GNB2L1 and SRC. Interacts with HIV-1 Tat. Binds to human echoviruses 1 and 8 capsid proteins and acts as a receptor for these viruses. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note= Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. TISSUE SPECIFICTY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in peripheral T-cells. Isoform beta-1C is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle). PTM: The cysteine residues are involved in intrachain disulfide bonds By similarity.SIMILARITY: Belongs to the integrin beta chain family.Contains 1 VWFA domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Integrin β1 Antibody, clone MB1.2 | MAB1997 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Rat View All»
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          © Merck Millipore代理MAB1997 Anti-Integrin β1 Antibody, clone MB1.2;抗整合素β 1抗体，克隆MB1.2store at +2℃ to +8℃，产品报价联系微信jinshanbio