货号：MAB1976B

品牌：Merck Millipore

规格：100Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated | MAB1976B View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits. View All» Immunogen: Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987). View All» Clone: LM609 View All» Isotype: IgG1 View All» Background Information: The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors. View All» Species Reactivity:

• Avian

• Bovine

• Canine

• Chicken

• Human

• Monkey

• Pig

• Rabbit
  View All» Non-Reactive Species:
  • Mouse

  • Rat
    View All» Application Notes: Immunoprecipitation: specifically immunoprecipitates the integrin alphaVbeta3 complex which, when resolved by SDS-PAGE under reducing conditions, results in an approximately 130 kDa band corresponding to the alphaV subunit and a 105 kDa band corresponding to the beta3 subunit (Cheresh, 1987). Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6 mm cryosections of chick chorioallantoic membrane fixed with acetone (Brooks, 1994). LM609 is also effective on 4% paraformaldehyde fixed, frozen tissues and cells. However it is not effective for immunohistochemical staining of paraffin-embedded tissue sections. Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL. Optimal working dilutions and protocols must be determined by end user. View All» Applications Not Recommended: Immunohistochemistry (Paraffin) View All» Control:
    • POSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995). NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV

    • Chen, 1995).
      View All» Purification Method: Protein A purfied View All» Presentation: Liquid in 0.01M PBS (pH 7.1), 0.1% sodium azide with 15 mg/mL BSA added as a stabilizer. View All» Storage Conditions: Maintain at 2–8°C in undiluted aliquots for up to 6 months. View All» UniProt Number: P06756 View All» Entrez Gene Number: NM_002210.2 View All» Gene Symbol:
      • ITGAV

      • DKFZp686A08142

      • MSK8

      • CD51

      • VNRA
        View All» Alternate Names: Vitronectin Receptor; CD51/CD61 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
        • Flow Cytometry

        • Immunohistochemistry

        • Immunoprecipitation

        • Affects Function
          View All» Entrez Gene Summary: ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. View All» UniProt Summary: FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.SIZE: 1048 amino acids; 116038 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. View All» Brand Family: Chemicon View All» Product Name: Anti-Integrin αVβ3 Antibody, clone LM609, biotin conjugated | MAB1976B View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Biotin View All» Host: Mouse View All»
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