货号：07-2072

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-DMAP1, C-terminus View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the C-terminal region of DMAP1. View All» Molecular Weight: ~53 kDa View All» Epitope: C-terminus View All» Immunogen: KLH-conjugated synthetic peptide corresponding to the C-terminal region of DMAP1. View All» Background Information: DNA methylation can help to regulate transcriptional silencing via repressive transcription complexes, which include methyl-CpG-binding domain proteins and histone deacetylases (HDACs). DNMT1, the core enzyme for mammalian DNA methylation, can also establish a repressive transcription complex consisting of DNMT1, HDAC2, and a third protein, termed DMAP1 for 'DNMT1-associated protein.' The 467-amino acid DMAP1 protein shares approximately 98% amino acid sequence homology with the mouse protein. DMAP1 interacts directly with the N-terminal region of DNMT1, and DMAP1 can repress transcription independently of histone deacetylase activity. DNMT1, HDAC2, and DMAP1 form a complex in vivo, and DMAP1 can interact directly with the transcriptional corepressor TSG101. The DNMT1-DMAP1 exists throughout the S phase; HDAC2 joins DNMT1 and DMAP1 only during late S phase. This provides a regulated means to deacetylate heterochromatin following replication. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human. Predicted to cross-react with mouse based on sequence homology. View All» Application Notes: ELISA: A 1:1,000 dilution of a previous lot was used in ELISA.Immunohistochemistry (Paraffin): A 1:50-100 dilution of a previous lot was used in immunohistochemistry. Immunocytochemistry: A 1:500 dilution of a previous lot was used. View All» Control: HL60 cell lysate View All» Quality Assurance: Evaluated by western blot on HL60 cell lysate.Western Blot Analysis: 1:100-1:500 dilution of this antibody detected DMAP1 in HL60 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified polyclonal antibody in buffer containing PBS with 0.09% (W/V) sodium azide. View All» Storage Conditions: Stable for 6 months at 2-8°C from date of reciept. For long term storage, store at -20°C.Handling Recommendations: Prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q9NPF5 View All» Entrez Gene Number: NP_061973 View All» Gene Symbol:
  • DKFZp686L09142

  • DNMAP1

  • DNMTAP1

  • EAF2

  • FLJ11543

  • KIAA1425

  • OTTHUMP00000008845

  • OTTHUMP00000008846

  • OTTHUMP00000008847

  • SWC4
    View All» Alternate Names:
    • DNA methyltransferase 1 associated protein 1

    • DNMT1 associated protein 1

    • DNMT1-associated protein 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)

      • ELISA

      • Immunocytochemistry
        View All» Entrez Gene Summary: This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionallyinactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. [provided byRefSeq]. View All» UniProt Summary: FUNCTION: Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. SUBUNIT STRUCTURE: Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, TCFL1/YL1 and YEATS4/GAS41. DMAP1 slso forms a complex with DNMT1 and HDAC2. Throughout S phase it interacts directly with the N-terminus of DNMT1, which serves to recruit DMAP1 to replication foci. DMAP1 interacts with ING1, a component of the MSIN3A transcription repressor complex, although this interaction is not required for recruitment of ING1 to heterochromatin. Interacts directly with the transcriptional corepressor TSG101. Interacts with the pro-apoptotic protein DAXX. SUBCELLULAR LOCATION: Nucleus. Note= Targeted to replication foci throughout S phase by DNMT1. SEQUENCE SIMILARITIES: Contains 1 SANT domain. View All» Product Name: Anti-DMAP1, C-terminus View All» Concentration: 0.25 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Rabbit View All»
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