货号：MAB1922

品牌：Merck Millipore

规格：100UL

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Laminin α2 Antibody, clone 5H2 | MAB1922 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with the 80 kDa fragment of the M-chain of human merosin. View All» Molecular Weight: 80 kDa View All» Immunogen: Purified human merosin View All» Clone: 5H2 View All» Isotype: IgG1 View All» Background Information: Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14 - 19 with multiple developmental abnormalities. The kidney phenotypes include avascular glomeruli, impaired branching morphogenesis, and renal agenesis. View All» Species Reactivity:

• Human

• Monkey

• Rabbit
  View All» Species Reactivity Note: Cross reacts with monkey and rabbit merosin. View All» Application Notes: ELISA: 50% maximal binding to human merosin at 1:50,000 dilution from a previous lot. Immunohistochemistry: 1:5,000 dilution from a previous lot was used for staining of 8 µm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody. Immunofluorescence: A previous lot of this antibody was used in immunofluorescent.Affinity chromatography: A previous lot of this antibody was used in IAP. Immunoprecipitation: A previous lot of this antibody was used in IP.Optimal working dilutions must be determined by end user. View All» Control: Vascular breast carcinomas, Cultured embryonic retinal neurons and RGCs View All» Quality Assurance: Routinely evaluated by Western Blot on Human Placenta lysate.Western Blot Analysis: 1:1000 dilution of this lot detected Laminin α2 (merosin) on 10 μg of Human Placenta lysate. View All» Purification Method: Unpurified View All» Presentation: Unpurified ascites in buffer containing no preservatives. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance. View All» UniProt Number: P24043 View All» Entrez Gene Number: NM_000426.3 View All» Gene Symbol:
  • LAMA2

  • LAMM
    View All» Alternate Names:
    • Laminin M chain

    • Merosin heavy chain

    • laminin M

    • laminin alpha 2 subunit

    • laminin, alpha 2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Cell Culture

      • Immunofluorescence

      • Immunohistochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P24043 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.SIZE: 3110 amino acids; 342771 Da SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (merosin) and laminin-4 (S- merosin).SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.TISSUE SPECIFICITY: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.DOMAIN: SwissProt: P24043 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular.DISEASE: SwissProt: P24043 # Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Laminin α2 Antibody, clone 5H2 | MAB1922 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Ascites View All» Host: Mouse View All»
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