货号：MAB1864

品牌：Merck Millipore

规格：100Ug

目录价：￥3977.00

市场价格：￥3380.45

会员价格：￥3181.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Tubulin Antibody, clone YL1/2 | MAB1864 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: MAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. View All» Molecular Weight: 55 kDa View All» Epitope: The epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). View All» Immunogen: Yeast tubulin View All» Clone: YL1/2 View All» Isotype: IgG2a View All» Background Information: Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Immunohistochemistry: 1:50-1:100 dilutoin from a previous lot was used on frozen sections.ELISA: 1:100-1:1,000 dilution from a previousl lot was used.Immunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation.Radioimmunoassy: A previous lot of this antibody was used on Radioimmuoassy.Optimal working dilutions must be determined by end user. View All» Control: All tissue View All» Quality Assurance: Routinely evaluated by Western Blot on A431 lysates.Western Blot Analysis: 1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide. View All» Storage Conditions: Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q71U36 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Immunohistochemistry

  • Immunoprecipitation

  • Radioimmunoassay

  • Western Blotting
    View All» UniProt Summary: FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.SUBUNIT STRUCTURE: Dimer of alpha and beta chains.TISSUE SPECIFICTY: Expressed at a high level in fetal brain. Ref.7PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively By similarity.DISEASE: Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem. Ref.7SIMILARITY: Belongs to the tubulin family. View All» Brand Family: Chemicon View All» Product Name: Anti-Tubulin Antibody, clone YL1/2 | MAB1864 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Rat View All»
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