货号：MAB1810

品牌：Merck Millipore

规格：100UL

目录价：￥5513.00

市场价格：￥4686.05

会员价格：￥4410.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ADAMTS-1 Antibody, clone 5D4E11B6 | MAB1810 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Clone 5D4E11B6 recognizes human ADAMTS-1. View All» Molecular Weight: 110 / 87 / 65 kDa View All» Epitope: Unknown View All» Immunogen: Myc-His carboxyl tagged full-length recombinant protein based on the human sequence. View All» Clone: 5D4E11B6 View All» Isotype: IgG View All» Background Information: ADAMTS-1 is a member of a family of extracellular proteases known as ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs). Members of the ADAMTS family share distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TSP) motif. Individual members of this family differ in the number of C-terminal TSP motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TSP motifs. ADAMTS-1 cleaves aggrecan, a cartilage proteoglycan and appears to demonstrate angiogenesis inhibitory activity. The maturation of ADAMTS-1 requires two independent and sequential processing events that may release two forms of the protein. With the cleavage of the prodomain from the 110 kDa zymogen, an 87 kDa active form remains which can be further processed by removal of the catalytic subunit from the TSP repeats, leaving the C-termianl 65 kDa soluble form. The proteolytic deletion of the last two TSP repeats, is probably significant for the in vivo function of this protein. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Reactivity with other species has not been tested. View All» Application Notes: Western blot: 1:2000 - 1:4000 dilution on A431 cell lysateNote: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms.Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate View All» Control:
  • A431 cell lysate

  • HEK293 cell lysate

  • PC-12 cell lysate

  • Embryonic mouse brain lysate

  • MCF-7 cell lysate
    View All» Presentation: Ascites. Liquid containing 0.05% NaN3. View All» Storage Conditions: Maintain at -20°C for up to 1 year from date of receipt. View All» UniProt Number: Q9UHI8 View All» Entrez Gene Number: NM_006988.3 View All» Gene Symbol:
    • ADAMTS1

    • KIAA1346

    • METH1

    • C3-C5
      View All» Alternate Names: METH-1 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. View All» UniProt Summary: FUNCTION: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. CATALYTIC ACTIVITY: Cleaves aggrecan at the 1938-Glu-|-Leu-1939 site, within the chondroitin sulfate attachment domain. COFACTOR: Binds 1 zinc ion per subunit (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). DOMAIN: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix. DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: The precursor is cleaved by a furin endopeptidase (By similarity). SIMILARITY: Contains 1 disintegrin domain and contains 1 peptidase M12B domain and contains 3 TSP type-1 domains. View All» Brand Family: Chemicon View All» Product Name: Anti-ADAMTS-1, clone 5D4E11B6 Monoclonal Antibody View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Mouse View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://16ao.com/thread-130225.htm
        © Merck Millipore代理MAB1810 Anti-ADAMTS-1 Antibody, clone 5D4E11B6;抗ADAMTS-1抗体，克隆5D4E11B6store at -20℃，产品报价联系微信jinshanbio