货号：MAB1618

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with all known forms of the Mr = 74,000 Dalton intermediate chain subunit (IC74) of mammaliam cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). View All» Molecular Weight: 74 kDa View All» Epitope: 74 kDa Intermediate chains, cytoplasmic View All» Immunogen: Bovine brain cytoplasmic dynein. View All» Clone: 74.1 View All» Isotype: IgG2b View All» Background Information: Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These protein "motors" contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile funtions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kDa), three intermediate chains (70-125 kDa), and at least four light chains (15-25 kDa). View All» Species Reactivity:

• Human

• Mouse

• Rat

• Bovine

• Drosophila

• Sheep

• Fish

• Xenopus
  View All» Species Reactivity Note: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid. Not tested on chicken, nematodes or Dictystelium. View All» Non-Reactive Species: Squid View All» Application Notes: Immunocytochemistry: 1:50-1:100 dilution from a previous lot was used. Reacts with cultured MDCK cells, NRR cells, N1E cells, and PTK-1 cells.Immunoprecipitation: 10 μL of a previous lot per 0.5 gm tissue was used. The monoclonal cleanly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530 kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.Immunoblotting: 1:1,000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Optimal working dilutions must be determined by end user. View All» Control: HeLa cells, A431 cell lysate View All» Quality Assurance: Routinely evaluated by Western Blot on A431 lysates.Western Blot Analysis: 1:500 dilution of this lot detected DYNEIN on 10 μg of A431 lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG2b in buffer containing 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. View All» Storage Conditions: Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. View All» UniProt Number: Q9UI46 View All» Entrez Gene Number: NM_012144.2 View All» Gene Symbol:
  • DNAI1

  • PCD

  • ICS

  • MGC26204

  • CILD1
    View All» Alternate Names:
    • Axonemal dynein intermediate chain 1

    • dynein intermediate chain DNAI1

    • dynein, axonemal, intermediate chain 1

    • dynein, axonemal, intermediate polypeptide 1

    • immotile cilia syndrome 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunofluorescence

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. This gene encodes an intermediate chain dynein, belonging to the large family of motor proteins. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome. View All» UniProt Summary: FUNCTION: SwissProt: Q9UI46 # Part of the dynein complex of respiratory cilia.SIZE: 699 amino acids; 79283 Da SUBUNIT: Consists of at least two heavy chains and a number of intermediate and light chains.DISEASE: SwissProt: Q9UI46 # Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:242650]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. & Defects in DNAI1 are a cause of Kartagener syndrome [MIM:244400]. Kartagener syndrome is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus.SIMILARITY: SwissProt: Q9UI46 ## Belongs to the dynein intermediate chain family. & Contains 5 WD repeats. View All» Brand Family: Chemicon View All» Product Name: Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://16ao.com/thread-130096.htm
        © Merck Millipore代理MAB1618 Anti-Dynein Antibody, 74 kDa Intermediate chains, cytoplasmic, clone 74.1;抗动力蛋白抗体，74 kDa 中间链，细胞质，克隆，产品报价联系微信jinshanbio