货号：3127

品牌：Merck Millipore

规格：

目录价：￥4319.00

市场价格：￥3671.15

会员价格：￥3455.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: LIGHT DIAGNOSTICS™ SimulFluor® RSV/Para 3, ~50 tests View All» Trade Name:

• LIGHT DIAGNOSTICS

• Chemicon (Millipore)
  View All» Qty/Pk: 2 mL View All» Product Overview: Intended Use The Light Diagnostics™ SimulFluor® RSV/Para 3 Immunofluorescence Assay is intended for the simultaneous detection and identification of respiratory syncytial virus (RSV) and/or parainfluenza 3 (para 3) following amplification in cell culture. For in vitro diagnostic use. Summary and Explanation Respiratory syncytial virus (RSV) occupies the genus Pneumovirus in the family Paramyxoviridae. It is a pleomorphic virus ranging from approximately 100 to 300 nm in diameter containing a linear, non-segmented, single-stranded, negative sense RNA. The envelope contains projections on the outer surface composed of two highly antigenic glycoproteins, F and G, and a major structural protein, N, in the nucleocapsid. The F, G, and N structural proteins divide RSV into two major antigenic groups; the "prototype" strains are Long (group A) and CH-18537 (group B) (1-4). RSV causes major epidemics of severe respiratory illness during the winter and spring seasons throughout the world. Because natural immunity to RSV is weak, these epidemics tend to occur every year or every other year. RSV can be isolated from nasopharyngeal aspirates, nose or throat swabs, or lung specimens taken within the first few days of illness. The virus grows in human epithelial cells such as HEp2, primary rhesus monkey kidney (RMK), A549, MRC-5, and NCI-H292. Rapid and direct tests on suitable clinical specimens are diagnostic of RSV infections provided that standardized reagent and adequate controls are used. Confirmation by virus isolation is desired wherever possible (5). Hospitalized RSV-infected infants have been successfully treated with ribavirin, administered as an aerosol. However, this requires specialized care, and is not given routinely (4). Immune globulin is also available for treatment. RSV vaccines have a long history of failure but are still under intense development (4). Parainfluenza 3 virus is a member of genus Paramyxovirus, also in the family of Paramyxoviridae (6). Parainfluenzaviruses are enveloped viruses, about 150-250 nm in diameter, with hemagglutinin neuraminidase (HN) and fusion (F) glycoproteins protruding through the viral membrane, and containing a single-stranded, negative-sence RNA (8-10). Human parainfluenzavirus type 3 (para 3) is one of 5 serotypes of paramyxovirus and is closely related to human mumps virus. Para 3 infections have similar, if not identical symptoms as RSV in young children. It causes croup, bronchiolitis and pneumonia in all age groups, with the primary infection in children being the most severe (5,8,10). Para 3 is responsible for more hospitalizations than parainfluenza 1 and 2 combined, and is second only to RSV as the etiologic agent for pneumonia in hospitalized children. It is important to identify the particular virus because of epidemiological considerations and avoid nosocomial spread of the virus. Para 3 causes widespread epidemics of "colds" and croup in all parts of the world, usually in the spring and at 1, 2, or 3 years intervals. Epidemics are particularly severe in populations with underlying risk factors such as malnutrition, overcrowding, vitamin A deficiency, cigarette smoke, or environmental toxins. Para 3 can be isolated from respiratory specimens in cultures of NCI-H292, RMK cells, and occasionally other cell lines depending on the particular strain of virus (8-11). Trypsin in the culture fluid at ~1.5 µg/mL concentration greatly aids in para 3 isolation in NCI-H292 cells. The cultures should be subpassaged at weekly intervals for a total of 3 passages to allow for viral recovery. Since definable cytopathology may not be evident, cultures must be tested by hemadsorption with guinea pig or human erythrocytes to access viral growth. Viruses can be serotyped by HA - hemodsorption, HI - hemagglutination inhibition, IFA - (immunofluorescence), IPA - (immunoperoxidase) and neutralization tests (8,10). Direct tests, including IFA, EIA, IPA, allow immediate typing of strains. Adequate specimens include nasal washes, throat swabs, and broncholaveolar lavages. Test Principle Light Diagnostics™ SimulFluor® RSV / Para 3 Immunofluorescence Assay utilizes a single reagent for the simultaneous detection and identification of RSV and parainfluenza 3. The primary component, specific for RSV, will bind to RSV F and G antigens in RSV-infected cells. The secondary component, specific for para 3, will bind to HN or F antigen in para 3 - infected cells. Unbound reagent is removed by rinsing with phosphate buffered saline (PBS). The antigen-antibody complexes can be visualized by fluorescence microscopy. When a fluorescein filter set is used, the RSV antigen-antibody complex will exhibit an apple-green fluorescence and the para 3 antigen-antibody complex will appear yellow-gold. Uninfected cells stain a dull red due to the presence of Evans blue in the reagent. View All» Key Applications: Immunofluorescence View All» Usage Statement:
  • For in vitro Diagnostic Use

  • CE Mark
    View All» Components:
    • SimulFluor® RSV/Para 3 Reagent- (Catalog No. 5240).

    • RSV Control Slides - (Catalog No. 5012).

    • Parainfluenza 1,2&3 Control Slides - (Catalog No. 5011).

    • Phosphate Buffered Saline (PBS) - (Catalog No. 5087).

    • Tween 20/Sodium Azide Solution (100X) - (Catalog No. 5037).

    • Mounting Fluid - (Catalog No. 5013).
      View All» Brand Family: Chemicon View All» Format: SimulFluor View All» Host: Mouse View All» Storage Conditions: When stored at 2° to 8°C, the SimulFluor® RSV/Para 3 Immunofluorescence Assay kit is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the kit expiration date. Warnings and Precautions * The sodium azide used as a preservative in the SimulFluor® RSV/Para3, PBS/Tween, and Mounting Fluid is toxic if ingested. Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. Upon disposal, flush with large volumes of water to prevent build-up in plumbing. * Pooling or alteration of any reagent may cause erroneous results. * Do not substitute reagents from other manufacturers. • Incubation times or temperatures other than those specified may give erroneous results. Any such change must be validated by the user. * Do not allow shell vials or slides to dry at any time during the staining procedure. * Handle all specimens and materials coming in contact with them as potentially infectious and dispose of them with proper precautions. Decontaminate with 0.05% sodium hypochlorite. * Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation. * Do not mouth pipette reagents. • Avoid contact with Evans Blue (present in SimulFluor® RSV/Para3 reagent) as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water. • Mounting Fluid contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water. View All» Detection Methods: Fluorescent View All» Product Name: LIGHT DIAGNOSTICS™ SimulFluor® RSV/Para 3, ~50 tests View All» Materials Required but Not Delivered: • Acetone, reagent grade; stored in glass • Deionized or distilled water • Virus culture controls (reference RSV and para 3 strains available from ATCC, Rockville, MD.) • Sodium hypochlorite solution, 0.05% (1:100 dilution of household bleach) • Cell lines susceptible to RSV and para 3 infections. Appropriate cell lines can be obtained from the American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville, MD 20852. • Tissue culture media such as RPMI or Eagle's Minimum Essential Medium (EMEM) with fetal bovine serum (FBS) and antibiotics, or equivalent • Viral transport medium which is non-inhibitory to RSV or para 3 (Hanks balanced salt solution (HBSS) with antibiotics, or equivalent) • Acetone-cleaned glass slides, non-fluorescing, with hydrophobic coating • Acetone, reagent grade or better • No. 1 coverslips • Aspirator device with disposable sterile Pasteur pipettes • Centrifuge capable of 700 to 950 x g with biohazard buckets and adaptors for shell vials • Fluorescence microscope with 100 watt mercury or halogen lamp, appropriate filter combination for fluorescein (excitation peak = 490 nm, emission peak = 520 nm), 160 to 200x and 400x magnification (dry objective) • Forceps • Humid chamber • Incubator, 37 + / - 1°C • Syringe and needle or other implement to remove coverslip from shell vial • Ultrasonic water bath • Vortex mixer or sonicator View All» Antibody Type: Monoclonal Antibody View All»
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