货号：MAB1219

品牌：Merck Millipore

规格：100Ug

目录价：￥4967.00

市场价格：￥4221.95

会员价格：￥3973.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CD14 Antibody, clone 2D-15C | MAB1219 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Product Overview: BIOCHEMISTRY: p I = 5.8 View All» Specificity: This antibody belongs to CD14 (assigned by the Third International Workshop on Leucocyte Differentiation Antigens, Oxford, 1986; McMichael et al. Eds., 1986). and reacts with 50 to 55kD protein. It will detect blood monocytes, Kupffer cells, red pulp macrophages, dendritic cells and also "epithelioid" or giant cells within granulomatous tissue (Hancock et al., 1983), foam cells in renal interstitium (Nolasco et al., 1984) and placental dendritic cells and macrophages (Barnett et al., 1984; Brooks et al., 1983; Polli et al., 1984; Hopper et al., 1986). Monocytes react with this antibody in the early stages of differentiation, promonocytes and monoblasts do not. Activity is not lost on monocyte/macrophage activation (Hancock et al., 1983). Cell reactivity Reacts with peripheral blood monocytes and weakly with granulocytes. Negative to platelets, erythrocytes and lymphocytes. Sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). Negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL). View All» Immunogen: Human peripheral blood mononuclear cells. View All» Clone: 2D-15C View All» Isotype: IgG1 View All» Species Reactivity: Human View All» Application Notes: This antibody is an important general cell marker for mononuclear phagocytes in normal samples and a variety of disease states including a proportion of cases of myeloid leukemia. It is suitable for flow cytometry, and immunoperoxidase staining on frozen tissue sections. SUGGESTED USAGE DILUTION 1. Flow cytometry and indirect immunofluorescence 1:25 Dilute with isotonic buffer. Use 50 μl per 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 μl buffer. 2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems. View All» Presentation: The purified antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry. View All» Storage Conditions: Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water. View All» UniProt Number: P08571 View All» Entrez Gene Number:

• NM_000591.2

• NM_001040021.1
  View All» Gene Symbol: CD14 View All» Alternate Names: LPS Receptor View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Flow Cytometry

  • Immunohistochemistry
    View All» Entrez Gene Summary: CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. View All» UniProt Summary: FUNCTION: SwissProt: P08571 # Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.SIZE: 375 amino acids; 40076 Da SUBUNIT: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.TISSUE SPECIFICITY: Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.SIMILARITY: SwissProt: P08571 ## Contains 11 LRR (leucine-rich) repeats. View All» Brand Family: Chemicon View All» Product Name: Anti-CD14 Antibody, clone 2D-15C | MAB1219 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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