货号：MAB1083

品牌：Merck Millipore

规格：100Ug

目录价：￥4438.00

市场价格：￥3772.30

会员价格：￥3550.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GFP Antibody, clone 3F8.2 | MAB1083 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: The antibody reacts with the 28 kDa GFP protein. View All» Molecular Weight: 28 kDa View All» Epitope: Full Length View All» Immunogen: Full length GFP protein. View All» Clone: 3F8.2 View All» Isotype: IgG1κ View All» Background Information: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. View All» Species Reactivity: Vertebrates View All» Species Reactivity Note: Reacts with most common vertebrate proteins containing the GFP tag. View All» Application Notes: Western Blot Analysis: 0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392). View All» Control: Western Blot: GFP recombinant protein View All» Quality Assurance: Routinely evaluated by Western Blot on GFP recombinant protein. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P42212 View All» Gene Symbol: GFP View All» Alternate Names: Green Fluorescent protein View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.SUBUNIT: Monomer.SPECIFICITY: Photocytes.PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.Biotechnological use Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.SIMILARITIES: Belongs to the GFP family.Biophysicochemical properties Absorption:Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.Abs(max)=395 nmHide | TopOntologies View All» Product Name: Anti-GFP Antibody, clone 3F8.2 | MAB1083 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»