货号：MAB10761

品牌：Merck Millipore

规格：EA

目录价：￥3828.00

市场价格：￥3253.80

会员价格：￥3062.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-AIF Antibody, clone 6C4F10 | MAB10761 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~ 57 kDa observed MW. AIF is synthesized as a precursor protein of 67 kDa and converted to a mature 57 kDa protein upon mitochondrial import and removal of the amino-terminal mitochondrial localization signal. View All» Immunogen: Histidine-tagged recombinant protein corresponding to human AIF. View All» Clone: 6C4F10 View All» Isotype: IgG1κ View All» Background Information: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami et al., 1999). Apoptosis inducing factor (AIF) gene was identified, and its encoded protein was shown to cause chromatin condensation and DNA fragmentation, typical hallmarks of apoptisis (Susin et al., 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF is highly conserved between human and mouse and widely expressed (Susin et al., 1999). View All» Species Reactivity: Human View All» Control: K562 cell lysate View All» Quality Assurance: Evaluated by Western Blot in K562 cell lysate.Western Blot Analysis: 1:500 dilution of this antibody detected AIF in 10 µg of K562 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Mouse monoclonal IgG1κ ascites containing 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: O95831 View All» Entrez Gene Number: NP_004199 View All» Gene Symbol:

• AIF

• PDCD8

• AIFM1
  View All» Alternate Names:
  • apoptosis-inducing factor, mitochondrion-associated, 1

  • striatal apoptosis-inducing factor

  • Programmed cell death protein 8

  • programmed cell death 8 (apoptosis-inducing factor)

  • programmed cell death 8
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro). Binds to DNA in a sequence-independent manner.COFACTOR: FAD.SUBUNIT STRUCTURE: Interacts with XIAP. SUBCELLULAR LOCATION: Mitochondrion intermembrane space. Nucleus. Note: Translocated to the nucleus upon induction of apoptosis. SEQUENCE SIMILARITIES: Belongs to the FAD-dependent oxidoreductase family. View All» Product Name: Anti-AIF, clone 6C4F10 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Ascites View All» Host: Mouse View All»
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