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Quality • Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing • Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process • Tested and found negative for bacteria, fungi, and mycoplasma • Protein concentrations are determined by Lowry method • Endotoxin units are measured by Limulus Amoebocyte Lysate assay • BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C • Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0 mm layer of BD Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Source Engelbreth-Holm-Swarm Mouse Tumor

Preparation and Storage Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.

Application • Cell Growth and Differentiation • Metabolism/Toxicology Studies • Invasion Assays • In Vitro and In Vivo Angiogenesis Assays • In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice