货号：701053

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：EACH

目录价：￥4600.00

市场价格：￥4140.00

会员价格：￥4140.00

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 This purified ABfinity™ Recombinant Rabbit Monoclonal Antibody recognizes epidermal growth factor receptor (EGFR [pY1068]) in human samples.ABfinity™ antibody characteristics include:• Validation in Western Blot, Immunofluorescence and Flow Cytometry• Superior lot-to-lot consistency• Extremely high specificity and sensitivity• Recombinant rabbit monoclonal antibodySpecific for EGFR Epidermal Growth Factor Receptor (EGFR), the receptor for the epidermal growth factor (EGF) family of proteins, is a175 kDa transmembrane glycoprotein belonging to the tyrosine kinase superfamily (Ref 1,2). Binding of EGFR to its ligands causes autophosphorylation of tyrosine kinase followed by activation of signal transduction in pathways connected to cell proliferation and differentiation (Ref 4). Tyrosine 1068, which lies within the cytoplasmic domain of the receptor, is an important autophosphorylation site that allows binding of Grb2 and activation of the Ras-Raf-ERK1/ERK2 signaling pathway (Ref 5). Deregulation of EGFR expression has been connected to carcinogenesis and metastasis (Ref 1,2), and EGFR has been a major focus for targeted cancer therapy (Ref 1-3).Exceptional Consistency Saves You Time and MoneyABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.High Specificity Means More Reliable DataThe proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in Western Blot, Immunofluorescence and Flow Cytometry.High Sensitivity Lets You Detect Low Levels of Target ProteinABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.Recombinant Rabbit IgG—Use Like Any Other IgG AntibodyAs with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Related Links:Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodiesSee Life Technologies' full line of ABfinity™ recombinant monoclonal antibodiesReferences:• Yarden Y. The EGFR family and its ligands in human cancer. Signalling mechanisms and therapeutic opportunities. Eur J Cancer. 2001 Sep;37 Suppl 4:S3-8. Review. PubMed PMID: 11597398• Wells A. EGF receptor. Int J Biochem Cell Biol. 1999 Jun;31(6):637-43. Review. PubMed PMID: 10404636• Saito T, Okada S, Ohshima K, Yamada E, Sato M, Uehara Y, Shimizu H, Pessin JE, Mori M. Differential activation of epidermal growth factor (EGF) receptor downstream signaling pathways by betacellulin and EGF. Endocrinology. 2004 Sep;145(9):4232-43. PubMed PMID: 15192046• Engström W. Differential effects of epidermal growth factor (EGF) on cell locomotion and cell proliferation in a cloned human embryonal carcinoma derived cell line in vitro. J Cell Sci. 1986 Dec;86:47-55. PubMed PMID: 3498731• Yamazaki T, Zaal K, Hailey D, Presley J, Lippincott-Schwartz J, Samelson LE. Role of Grb2 in EGF-stimulated EGFR internalization. J Cell Sci. 2002 May 1;115(Pt 9):1791-802. PubMed PMID: 11956311 规格

Reactivity:	Human
Validated Application:	Flow Cytometry,Immunofluorescence,Western Blot
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Monoclonal
Clone ID:	2H9L3
Antibody Fragment:	Whole Antibody
Target Gene:	EGFR [pY1068]
Gene ID:	1956
Gene Symbol:	EGFR
Accession Number:	P00533
Label or Dye:	Unconjugated
Purification:	Purified
Concentration:	0.5 mg⁄ml
Form:	Liquid
Neutralizing Antibody:	No
Product Size:	100 µg
Protein Family:	Growth Factors & Receptors
Research Category:	Signal Transduction
Shipping Condition:	Wet Ice

内容及储存 2 - 8°C for up to 1 month -20°C for long storage Avoid repeated freezing and thawing.