货号：700050

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥4692.00

市场价格：￥4222.80

会员价格：￥4222.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Smad2 [pT8] ABfinity™ Recombinant Rabbit Monoclonal Antibody is validated to react with human and has an expected reactivity with bovine, rat, drosophila, xenopus, and mouse. This antibody is validated for use in Western Blot, Immunofluorescence, Flow Cytometry and ChIP. Smad2 [pT8] is encoded by the 4087 gene, also known as Mad-related protein 2, MADH2 protein, Mad2 protein. SMAD2, also known as MADH2 or MAD2 regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation (1). Smad2 interacts with the TGF-beta receptors (2, 3) through its interaction with the SMAD anchor for receptor activation (SARA) protein. The regulation of Smad2 phosphorylation on threonine 8 by ERK1 (4-7) and calmodulin is critical for Smad2-mediated signaling. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors and thereby participating in the regulation of gene expression. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGFβ signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal-regulated kinase 1 (ERK1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin1. Cao, Z., et al. (2003) Levels of phospho-Smad2⁄3 are sensors of the interplay between effects of TGF-beta and retinoic acid on monocytic and granulocytic differentiation of HL-60 cells. Blood. 101(2):498-507.2. Cordenonsi, M., et al. (2003) Links between tumor suppressors. p53 Is required for TGF-beta gene responses by cooperating with Smads. Cell. 113(3):301-314.3. Hayashida, T., et al. (2003) Cross-talk between ERK MAP kinase and Smad signaling pathways enhances TGF-beta-dependent responses in human mesangial cells. FASEB J. 17(11):1576-1578. 4. Funaba, M., et al. (2002) Modulation of Smad2-mediated signaling by extracellular signal-regulated kinase. J. Biol. Chem. 277(44):41361-41368. 5. Blanchette, F., et al. (2001) Cross-talk between the p42⁄p44 MAP kinase and Smad pathways in transforming growth factor beta 1-induced furin gene transactivation. J. Biol. Chem. 276(36):33986-33994. 6. Brown, J.D., et al. (1999) MEKK-1, a component of the stress (stress-activated protein kinase⁄c-Jun N-terminal kinase) pathway, can selectively activate Smad2-mediated transcriptional activation in endothelial cells. J. Biol. Chem. 274(13):8797-8805. 7. Nakao, A., et al. (1997) Identification of Smad2, a human Mad-related protein in the transforming growth factor beta signaling pathway. J. Biol. Chem. 272(5):2896-2900. 规格

Reactivity:	Human,Mouse,Rat,Xenopus,Drosophila,Bovine
Validated Application:	Flow Cytometry,Western Blot,Chromatin Immunoprecipitation,Immunofluorescence
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Monoclonal
Clone ID:	11H10L30
Antibody Fragment:	Whole Antibody
Target Gene:	SMAD2 [pT8]
Gene ID:	4087
Gene Symbol:	Smad2
Accession Number:	Q15796
Label or Dye:	Unconjugated
Target Modification State:	Phospho
Purification:	Protein A⁄G Affinity Chromatography
Color:	Unconjugated
Form:	Liquid
Mass:	100 µg
Neutralizing Antibody:	No
Product Size:	100 µg
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.