货号：61011

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：KIT

目录价：￥7529.00

市场价格：￥6776.10

会员价格：￥6776.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The Dynabeads® mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications. Advantages of the Dynabeads® mRNA DIRECT™ Kit:• Fast—15 minute procedure yields pure, intact mRNA• Highly pure mRNA isolation—best choice upstream of cDNA synthesis• Sensitive mRNA isolation—enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)System overviewThe isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)₂₅ residues covalently coupled to the surface of the Dynabeads® . Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol). 1 mg of Dynabeads® Oligo (dT)₂₅ beads (200 µL) binds up to 2 µg of mRNA. A typical mammalian cell contains about 10–30 pg of total RNA, from which 1–5% is mRNA. 规格

Sample Type (General):	Liquid Samples (e.g. Serum),Yeast,Blood,Tissue,Viral Samples,Plant Samples,Cells,FFPE & Fixed Samples,RNA
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	RT-PCR (Endpoint),Nuclease Protection Assays,Northern Blotting,Cloning,Real-Time PCR,cDNA Library Construction
Product Size:	5 mL
Final Product:	mRNA
Isolation Technology:	Magnetic Bead
Shipping Condition:	Room Temperature

内容及储存 Contents:5 ml Dynabeads oligo (dT)₂₅30 ml Lysis/Binding Buffer60 ml Washing Buffer A30 ml Washing Buffer B15 ml 10 mM Tris-HCl (Elution buffer)Contains enough reagents for 20 standard isolations. Store at 2 to 8°C.