货号：46300018

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：2X1ML

目录价：￥337.00

市场价格：￥303.30

会员价格：￥303.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme. A T4 DNA Ligase Technical Bulletin is available.Applications: Cloning (blunt-end or cohesive-end ligation) (2). Adding linkers or adapters to blunt-ended DNA (2).Source: Purified from E. coli œ lysogen NM989.Performance and Quality Testing: Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested.Unit Definition: One unit catalyzes the exchange of 1 nmol ³²P-labeled pyrophosphate into ATP in 20 min. at 37°C. (One unit is equal to approximately 300 cohesive-end ligation units.)Unit Reaction Conditions: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl₂ , 10 mM DTT, 66 µM ATP, 3.3 µM 32 P-labeled pyrophosphate, and enzyme in 0.1 ml for 20 min. at 37°C. 规格

Product Size:	2 x 1 mL
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl₂ , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.