货号：4451893

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：EA

目录价：￥9458.00

市场价格：￥8512.20

会员价格：￥8512.20

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from Tempus™ Blood RNA Tubes using proven magnetic bead-based technology. A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis. • Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit • Simple magnetic bead workflow—no spin columns• Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitorsMaximize Total RNA Yields From Blood SamplesThe MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from Tempus™ Blood RNA Tubes. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can recover 3-25 µg from a single Tempus™ Blood RNA tube. A TURBO DNase™ step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible. The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from Tempus™ Blood RNA tubes as shown by TaqMan® gene expression assays. Users are strongly advised to perform appropriate validation studies against their miRNA targets.Flexible Purification Formats The MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods. • For low-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5mL microfuge tubes with the bead?RNA-complex remaining in a single processing tube until the final elution step. • For medium-to-high–throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process. • For high-throughput users, the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing. Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps. The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX™ Express-96 Magnetic Particle Processor (deep well version only). Rapid Processing Time Processing times will vary based on format selected, but twelve Tempus™ Blood RNA tubes can be processed manually with the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours.An Alternative to Full PurificationFor users who perform primarily TaqMan®-based real-time RT-PCR experiments, and do not need to archive RNA⁄cDNA, an alternative to the MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR. This kit makes it possible to perform expression analysis directly from Tempus™ Blood RNA tubes without conventional RNA purification. One-Stop ShoppingMagMAX™ for Stabilized Blood Tubes RNA Isolation Kit is compatible with the suite of Life Technologies’ reverse transcription mixes (SuperScript®), PCR master mixes (both TaqMan® and SYBR® based), and other blood workflow products. Simplify the ordering process and reduce freight cost by getting more from one supplier.Specifications:• Yield (Range): 3 - 25µg • Quality (RIN) ): ≥ 8• Quality (A260⁄280) ): 1.8 – 2.2 • gDNA (w⁄w) ): <1.56% • Equipment Needed : *Refrigerated centrifuge (4°C) with swing bucket rotor capable of 5,000 x g with *50 mL conical tube adapters *50ml conical tubes *For tube format users: Tabletop microcentrifuge or picocentrifuge• Number of Preps: 96 preparations (blood tubes)NOTE: Blood tubes and magnetic stands are sold separately.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Sample Type (General):	Blood
High Throughput Compatibility:	Automated Protocols,Not High Throughput-Compatible (Manual),High Throughput-Compatible
Downstream Application:	Real-Time PCR
Format:	Tube(s),96-well plate
Product Size:	96 preps
Final Product:	Total RNA
Yield (up to):	25 µg
Purification Time:	1.9 hrs
Isolation Technology:	Magnetic Bead

内容及储存 Kit ComponentsBox 1:Store at RT• Tempus® Resuspension Solution, 12 mL• Binding Solution Concentrate 5 mL • Wash Solution 1 Concentrate, 2 x 16mL (Before using the Wash Solution 1 Concentrate, add the correct volume of isopropanol (user-supplied), as indicated on the bottle)• Wash Solution 2 Concentrate, 30 mL (Before using the Wash Solution 2 Concentrate, add the correct volume of ethanol (user-supplied), as indicated on the bottle)• Elution Buffer 10 mL• Processing Plate (96-well) 1 plateStore at 2 to 8 °C, Do not freeze.• Tempus® Pre-Digestion Wash, 2 x 200 mL• RNA Binding Beads, 2 mL • Tempus® 1X PBS, 2 x 150 mLBox 2:Store at -15 to -25 °C• Tempus® Proteinase, 0.25 mL• TURBO™ DNase, 1 mL