货号:4398968
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:EACH
目录价:¥22378.40
市场价格:¥20140.56
会员价格:¥20140.56
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
The Applied Biosystems® TaqMan® Rodent MicroRNA B Array v2.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 133 and 77 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan® MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details of the included assays is provided in the Array Map file. Purchase of the Megaplex™ RT Primers, Rodent Pool A is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool A for situations where sensitivity is of the utmost importance and/or sample is limiting.TaqMan® MicroRNA Arraysprovide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan® MicroRNA Assays in parallel.Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the the TaqMan® MicroRNA Array v2.0. When sensitivity is of the utmost importance, and/or sample is limiting Megaplex™ PreAmp Primers provide an optional preamplification step. TaqMan® MicroRNA Arrays v2.0 and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with Sanger miRBase v10 to be created in as little as 5 hours, providing the ideal miRNA profiling solution.Simplify Sample Handling and Increase Sample ThroughputFollowing reverse transcription of miRNA targets using Megaplex RT Primers and optional preamplification using Megaplex PreAmp Primers, TaqMan® Universal PCR Master Mix is simply combined with each reaction and pipetted into each of the eight sample loading ports in the TaqMan® Array, simplifying sample handling and increasing sample throughput.Provides Comprehensive Coverage of Sanger miRBase v10 Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan® MicroRNA Arrays v2.0. The content is derived from miRBase v10, and provides close to full coverage of Sanger with the most up-to-date TaqMan® MicroRNA Assays. Each array (either human or rodent) contains a selection of carefully chosen endogenous control assays, providing you with a variety of options for data normalization. Of particular note is the inclusion of a new mammalian U6 assay, repeated four times on each card, and an assay unrelated to mammalian species, ath-miR159a, providing a process control.Ideal for miRNA ProfilingThe highly streamlined workflow—coupled with the high specificity, sensitivity, and dynamic range of TaqMan® Assays—make this product solution ideal for miRNA profiling, especially when compared to microarrays.
详细说明
常用规格
Form: |
Dried Down
|
Format: |
384-well microfluidic card
|
Species: |
Rat,
Mouse
|
Label or Dye: |
FAM™
|
Product Size: |
4 pack
|
Reaction Speed: |
Standard
|
Research Category: |
Gene Regulation,
Epigenetics
|
Internal Probe Modification: |
Non-Fluorescent Quencher (3'),
MGB (Minor Groove Binder) (3'),
FAM (5')
|
Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures.
|
内容和储存
Contents: 384-well arrays and information CD. Storage: The TaqMan® MicroRNA Arrays are shipped at ambient temperature. Upon arrival, store arrays at 2 to 8 ° and keep them protected from light.
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