货号：4392653

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：40 RXN

目录价：￥2349.00

市场价格：￥2114.10

会员价格：￥2114.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The Applied Biosystems® TaqMan® RNA-to-C_T™ 1-Step Kit delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems®Õ comprehensive library of TaqMan® Gene Expression assays. The TaqMan® RNA-to-C_T™ 1-Step Kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.• Accurate across a wide dynamic range for dependable performance • High sensitivity, enabling low copy number detection and precise quantification • Validated with TaqMan® Gene Expression Assays for easy experimental setup• Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences• Fewer pipetting steps means less hands-on-time and less chance for errorsEfficiency across a Broad Range of Input ConcentrationsT™ 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan® One-Step RT-PCR Master Mix Reagents.High Sensitivity at Low Target Concentration The sensitivity of TaqMan® RNA-to-C_T™ 1-Step Kit was validated using a synthetic RNA template for which copy number is precisely known. Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates. Figure 2 shows the expected quantity of target and corresponding mean CT values. Statistical analysis indicates high confidence of sample quantification based on a T-test (Table I), consistent with as few as 10 copies of target. 规格

PCR Method:	1-Step qRT-PCR
Purity or Quality Grade:	UP (Ultra Pure)
Reverse Transcriptase:	ArrayScript™ UP
Polymerase:	AmpliTaq Gold® DNA Polymerase
Detection Method:	Primer-Probe Detection
Primer-Probe Compatibility:	TaqMan®
Reaction Speed:	Standard
Platform:	7300 System,7500 System,StepOnePlus™, Standard Mode,StepOne™, Standard Mode,7900HT System,7000 System,7700 System
Sample Type (General):	RNA
Passive Reference Dye:	ROX (Pre-mixed)
Product Size:	40 reactions
Shipping Condition:	Wet Ice

内容及储存 Sufficient for 40 reactions at 50 µL reaction volume. Includes: • 1 x 1 mL tube of 2X master mix containing AmpliTaq Gold® DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening. • 1 x 50 µL tube of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15° to -30°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).