货号:4389986
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:200 RXN
目录价:¥9007.00
市场价格:¥8106.30
会员价格:¥8106.30
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR® Green RNA-to-CT™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes: • AmpliTaq® Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA • An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals • RNase inhibitor, to prevent degradation of RNA templates • A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems® real-time PCR instruments.
规格
PCR Method: |
1-Step qRT-PCR |
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Purity or Quality Grade: |
UP (Ultra Pure) |
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Reverse Transcriptase: |
ArrayScript™ UP |
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Polymerase: |
AmpliTaq Gold® DNA Polymerase |
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Detection Method: |
SYBR |
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Primer-Probe Compatibility: |
Unlabeled Oligos |
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Reaction Speed: |
Standard |
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Platform: |
7300 System,7500 System,StepOnePlus™, Standard Mode,StepOne™, Standard Mode,7900HT System,7000 System,7700 System |
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Sample Type (General): |
RNA |
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Passive Reference Dye: |
ROX (Pre-mixed) |
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Label or Dye: |
SYBR® Green |
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Product Size: |
200 reactions |
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Shipping Condition: |
Wet Ice |
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内容及储存
Sufficient for 200 reactions at 50 µL reaction volume and includes: • 1 x 5 mL tube of 2X master mix containing SYBR®Green 1 Dye, AmpliTaq Gold® DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening. • 1 x 80 µL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
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