货号：4374966

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：(200 RXN)

目录价：￥6994.00

市场价格：￥6294.60

会员价格：￥6294.60

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each).The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives. Features of this kit include:• Linear target amplification for real-time PCR• Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost• 10-fold greater dynamic range than other kitsExtensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA. 规格

Reverse Transcriptase:	MultiScribe™ MuLV
Downstream Application:	RT-PCR (Endpoint),Real-Time PCR
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	200 reactions
Final Product:	cDNA (First-Strand)
Final Product Size(s):	7 kb or less
Optimal Reaction Temperature:	37° C

内容及储存 Contents: • 10X RT Buffer (1 mL) • 10X RT Random Primers (1 mL) • 25X dNTP Mix (100 mM, 0.2 mL) • MultiScribe® Reverse Transcriptase (0.2 mL at 50 U/µL) • RNase Inhibitor (2 × 0.1 mL at 20 U/µL) Store between -15°C to -25°C.