货号：4328217

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：500 UNITS

目录价：￥5356.05

市场价格：￥4820.45

会员价格：￥4820.45

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The Applied Biosystems® GeneAmp® High Fidelity PCR System is designed for accuracy and increased PCR yield.• AmpliTaq® DNA Polymerase-based enzyme blend is formulated for increased fidelity and yield of PCR products• Proofreading activity enables amplification of DNA fragments up to 10 kb• Two-buffer system provides versatilityHigh FidelityThe GeneAmp® High Fidelity PCR System contains an enzyme blend of AmpliTaq® DNA Polymerase and a new thermo stable proofreading enzyme. This proprietary blend increases the fidelity of PCR 3-fold over Taq DNA Polymerase. The careful blending of these enzymes prevents truncated products resulting from misincorporated nucleotides, and limits primer degradation by the 3' to 5' exonuclease activity of the proofreader. The increased sensitivity of the unique GeneAmp® High Fidelity Enzyme Mix enables detection down to 1 ng of complex templates such as human genomic DNA.Flexible KitThe system is supplied with buffers specifically designed to enhance fidelity, sensitivity, and yield of PCR experiments. Two versions of the buffer are included for flexibility. For ease of use, one version contains a final MgCl₂concentration of 1.5 mM. The second buffer does not contain MgCl_2. A separate MgCl₂ solution is also provided for optimizing MgCl₂ concentration. The system allows for amplification of products ranging from a hundred bases up to 10 kb from human genomic DNA. It can be used with templates from various sources such as mammalian genomic DNA, artificial chromosomes, plasmids, and cDNA.Note:See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for human or animal therapeutic or diagnostic use. 规格

Polymerase:	AmpliTaq® DNA Polymerase
Hot Start:	No
Fidelity (vs. Taq):	3 X
Reaction Format:	Separate Components
Product Overhang:	Blunt
Reaction Speed:	Standard
Final Product Size(s):	10 kb or less
Product Size:	200 reactions
Exonuclease Activity:	5' - 3',3' - 5'

内容及储存 Contains GeneAmp® High Fidelity Enzyme Mix, 10X PCR Buffer with MgCl₂, 10X PCR Buffer without MgCl₂, and MgCl₂ solution. Sufficient for 200 PCR amplifications, 50 µl each. Store at –20°C.