货号：4313803

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：MASTER MIX

目录价：￥95277.60

市场价格：￥85749.84

会员价格：￥85749.84

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Perform high-throughput real-time RT-PCR in a single step with the TaqMan® One-Step RT-PCR Master Mix Reagents from Life Technologies. This TaqMan® One-Step RT-PCR Master Mix Reagents Kit (10-pack) combines reverse transcription and cDNA amplification steps into one reaction tube, simplifying your real-time PCR experiments. The kit is ideal for all high-throughput applications, because setup is quick, easy, and eliminates the risk of sample crossover and contamination.This TaqMan® One-Step RT-PCR Master Mix Reagent Kit includes:• AmpliTaq Gold® DNA Polymerase: A chemically modified hot-start DNA polymerase that prevents non-specific amplification while providing maximum efficiency• MultiScribe™ Reverse Transcriptase: Provides longer cDNA and higher yield• RNase Inhibitor: Prevents degradation of RNA templates during the reverse transcription step• Passive Reference I: Normalizes signal and corrects inter-well signal variationEverything in One Kit:Each 10-pack includes 10 TaqMan® One-Step RT-PCR Master Mix Reagents Kits, each of which includes one tube of MultiScribe™ Reverse Transcriptase/RNase Inhibitor Mix (40X) for reverse transcription, and one tube of TaqMan® Universal PCR Master Mix, No AmpErase® UNG (2X) for DNA amplification. Both tubes are pre-mixed for minimal reagent handling and convenience. Just add total or poly-(A)+ RNA, TaqMan® probes, and gene-specific primers for both reverse transcription and amplification. That's all there is to it. 详细说明 常用规格

Contains 10 x vial 1 (2X AmpliTaq Gold® DNA Polymerase mix, dNTPs + dUTP, Passive Reference 1, and buffer) and 10 x vial 2 (40X RT enzyme mix with MultiScribe™ RT and RNase Inhibitor. Store at 2-8°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).