货号：4304134

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：10 PK

目录价：￥69425.00

市场价格：￥62482.50

会员价格：￥62482.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 TaqMan® Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility. Advantages of TaqMan® Reverse Transcription Reagents:• Contain MultiScribe® Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)• Random hexamers and oligo (dT)₁₆ included for flexibility• Individual components provide versatility in assay set-up• Compatible with one-step or two-step RT-PCR reactionsUsing TaqMan® Reverse Transcription ReagentsTaqMan® Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.Built-in normalizationThe TaqMan® reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube. 规格

Reverse Transcriptase:	MultiScribe™ MuLV
Downstream Application:	Real-Time PCR
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	2,000 reactions
Final Product:	cDNA (First-Strand)
Final Product Size(s):	5 kb or less
Optimal Reaction Temperature:	42° C

内容及储存 Contents: MultiScribe™ Reverse Transcriptase (100 µL at 50U/µL). Store for up to one year at -20°C.