货号：18265017

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：2.0ML (4 X 0.5ML) #18265

目录价：￥829.00

市场价格：￥746.10

会员价格：￥746.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 10⁶ cfu/µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.Features of Subcloning Efficiency™ DH5α™ Competent Cells:• Designed for general, everyday use• Contain genetic markers useful for general applicationsIdeal for routine subcloning proceduresSubcloning Efficiency™ DH5α™ Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 10⁶ transformants/µg control DNA per 50 µL reaction.Flexible cloning capabilitiesSubcloning Efficiency™ DH5α™ Competent Cells contain the following genetic markers resulting in these benefits:• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal• recA1 ensures increased insert stability and prevents unwanted recombination• endA1 improves the yield and quality of plasmid DNA prepared from minipreps• DH5α™ competent cells support replication of M13mp vectors but do not support plaque formationGenotype: F^- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_k^-, m_k⁺) phoA supE44 thi-1 gyrA96 relA1 λ^- 规格

Competent Cell Type:	Chemically Competent
Species:	E. coli
Format:	Tube(s)
Transformation Efficiency:	> 1x10^6
Bacterial or Yeast Strain:	DH5α™
Product Size:	40 reactions
Blue-White Screening:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Reduces Recombination:	Yes
Cloning Methylated DNA:	No
Improves Plasmid Quality:	Yes
Propagating ccdB Vectors:	Not for ccdB vector propagation
F' Episome (to make ssDNA):	Lacks F' Episome
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA):	No
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Shipping Condition:	Dry Ice

内容及储存 Contains: • Subcloning Efficiency™ DH5α™ Competent Cells: 4 vials, 500 µl each (total of 2 ml) • pUC19 DNA (100 pg/ul): 1 vial, 20 µl Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.