货号：18054015

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UN

目录价：￥2123.00

市场价格：￥1910.70

会员价格：￥1910.70

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from ³H-poly(dU) in 1 h at 37°C.Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl₂ , 4 µg/ml ³H-poly(dU)n at 7 x 10 5 cpm/µg, and 1 unit ofenzyme in 50 µl for 10 min. at 37°C (4). 规格

Enzyme:	UDG
Product Size:	100 units
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Store in freezer (-5 to -30°C).