货号：18033019

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：2500 UN

目录价：￥4371.00

市场价格：￥3933.90

会员价格：￥3933.90

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.Application: Synthesis of labeled and unlabeled RNA transcripts (1).Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid.Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in atranscription reaction.Unit Definition: One unit hydrolyzes 1 nmol of ribonucleotide into acid-precipitable material in 1 h at 37°C using a T7 transcriptionvector as template.Unit Reaction Conditions: 40 mM Tris-HCl (pH 8.0), 25 mM NaCl, 8 mM MgCl₂ , 2 mM spermidine-(HCl)₃ , 5 mM DTT, 0.4 mM ATP, 0.4 mM CTP, 0.4 mM GTP, 0.4 mM UTP, 1 µCi [³H]GTP, 1 µg Sph I-cut pT7L13, and enzyme in 50 µl for 10 min. at 37°C. 规格

Promoter:	T7
Hot Start:	No
Polymerase:	T7 RNA Polymerase
Product Size:	2500 units
Shipping Condition:	Wet Ice

内容及储存 T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl₂ , 10 mM spermidine-(HCl)₃ , 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.