货号：18018010

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：500U

目录价：￥2295.00

市场价格：￥2065.50

会员价格：￥2065.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 SP6 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage SP6 promoter sequences. This enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from an SP6 promoter. An SP6 RNA Polymerase Technical Bulletin is available.Application: Synthesis of labeled and unlabeled RNA transcripts (1,2).Source: Purified from E. coli expressing the SP6 RNA polymerase gene on a plasmid.Performance and Quality Testing: 3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in atranscription reaction.Unit Definition: One unit incorporates 1 nmol of ribonucleotide into acid-precipitable material in 1 h at 37°C using an SP6 transcription vector as template.Unit Reaction Conditions: 40 mM Tris-HCl (pH 7.9), 6 mM MgCl₂ , 2 mM spermidine-(HCl)₃ , 1 mM DTT, 0.4 mM ATP, 0.4 mM GTP,0.4 mM CTP, 0.4 mM UTP, 1 flCi [ 3 H]GTP, 1 µg Pvu I-cut pSV•SPORT 1, and enzyme in 50 µl for 10 min. at 37°C. 详细说明 常用规格

Promoter:	SP6
Hot Start:	No
Polymerase:	SP6 RNA Polymerases
Product Size:	500 units
Shipping Condition:	Wet Ice
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

内容和储存

SP6 RNA Polymerase is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 7.9), 30 mM MgCl₂ , 10 mM spermidine-(HCl)₃], vial of 10 mM DTT. Store at -20°C.