货号：18012021

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UN

目录价：￥2926.00

市场价格：￥2633.40

会员价格：￥2633.40

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities. Applications:Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.Source:Purified from E. coli expressing the Klenow fragment on a plasmid.Performance and Quality Testing:SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.Unit Definition:One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer. 规格

Hot Start:	No
Polymerase:	DNA Polymerase I
Product Size:	100 units
Exonuclease Activity:	3' - 5'
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 DNA Polymerase I, Large (Klenow) Fragment is supplied with a vial of 10X REact® 2 Buffer [500 mM Tris-HCl (pH 8.0), 100 mM MgCl₂ , 500 mM NaCl], vial of dilution buffer. Store at -20°C.