货号：18010017

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：250 UN

目录价：￥2215.00

市场价格：￥1993.50

会员价格：￥1993.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.• Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs• Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmidPerformance and quality testing Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.Unit definition One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.Unit reaction conditions 50 mM potassium phosphate (pH 7.0), 6.7 mM MgCl₂ , 1 mM 2-mercaptoethanol, 80 µg/mL template/primer, 32 µM dTTP, 69 nM [³H]dTTP, and enzyme in 100 µL for 30 min at 37°C. 规格

Hot Start:	No
Polymerase:	DNA Polymerase I
Product Size:	250 units
Exonuclease Activity:	5' - 3',3' - 5'
Shipping Condition:	Wet Ice

内容及储存 Store in freezer (-5 to -30°C).