货号：18004028

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：1000 UN

目录价：￥4267.00

市场价格：￥3840.30

会员价格：￥3840.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 T4 Polynucleotide Kinase catalyzes the transfer of the γ -phosphate of ATP to the 5´ hydroxyl terminus of DNA or RNA.A T4 Polynucleotide Kinase Technical Bulletin is available. Applications: 5´ end-labeling of DNA or RNA (1). Phosphorylation of synthetic linkers (1).Source: Purified from E. coli expressing the T4 polynucleotide kinase gene in a λ vector.Performance and Quality Testing: Ribonuclease, endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease, and phosphatase assays;labeling efficiency tested.Unit Definition: One unit transfers 1 nmol of phosphate of ATP to the 5´ hydroxyl termini of micrococcal nuclease-treated DNA in30 min. at 37°C.Unit Reaction Conditions: 70 mM Tris-HCl (pH 7.6), 0.1 M KCl, 10 mM MgCl₂ , 1 mM 2-mercaptoethanol, 0.3 mM [γ-³²P]ATP,0.38 mg/ml micrococcal nuclease-treated DNA, and enzyme in 150 µl for 30 min. at 37°C. 规格

Enzyme:	T4 Polynucleotide Kinase
Product Size:	1,000 units
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 T4 Polynucleotide Kinase is supplied with a vial of 5X forward reaction buffer [350 mM Tris-HCl (pH 7.6), 50 mM MgCl₂ , 500 mM KCl, 5 mM 2-mercaptoethanol] and a vial of 5X exchange reaction buffer [250 mM imidazole-HCl (pH 6.4), 60 mM MgCl₂ , 5 mM 2-mercaptoethanol, 350 µM ADP]. Store at -20°C.