货号：15440019

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：6000 UN

目录价：￥1135.00

市场价格：￥1021.50

会员价格：￥1021.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-GGGCC↓C-3´ 3´-C↑CCGG G-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	15 U/ µL
Contents	Apa I 10X Buffer L (1mL) 10X Loading Buffer (1mL)
Reaction Temperature	37°C
Reaction Mixture	Apa I 1 µL 10X Buffer L 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	Enzyme is inactivated by heating at 65°C for 20 minutes.
Star Activity
Effect of DNA Methylation	Enzyme activity is affected by dcm methylase or CG methylase depending on the sequence following the recognition site.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer L	100 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol
Source	Acetobacter pasteurianus sub. pasteurianus

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	100	<20	<20	<20	<20

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Enzyme:	Apa I
Product Size:	6000 units
Concentration:	15 U⁄µl
Compatible Buffer:	10X Buffer L
Methylation Sensitivity:	CpG methylation-sensitive,dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5100 mM KCl0.1 mM EDTA1 mM Dithiothreitol (DTT)0.15% Triton® X-1000.01% BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.