货号：15242019

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UN

目录价：￥361.00

市场价格：￥324.90

会员价格：￥324.90

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-GmA↓TC-3´ 3´-CT↑mAG-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	10 U/ µL
Contents	Dpn I 10X Buffer T (1mL) 10X Loading Buffer (1mL)
Reaction Temperature	37°C
Reaction Mixture	Dpn I 1 µL 10X Buffer T 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	Enzyme is inactivated by heating at 70°C for 15 minutes.
Star Activity	Unrelated site may be cut under conditions of low ionic strength, high enzyme concentration, or glycerol concentration >5%.
Effect of DNA Methylation	Enzyme can cut the sequence GmATC (A is methylated) and cannot cut the sequence GATC (A is not methylated). This enzyme can cut DNA prepared from E. coli strain (dam+ strain) commonly used, but not PCR product. Enzyme activity is not affected by dam methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of pBR322 DNA (prepared from dam+ strain) in 50 µL of the reaction mixture in 1 hour at 37°C.
10X Buffer T	330 mM Tris acetate, pH 7.9 100 mM Magnesium acetate 5 mM Dithiothreitol (DTT) 660 mM Potassium acetate
Source	Escherichia coli carrying the plasmid encoding the Dpn I gene.

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	60	60	120	140	100

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Enzyme:	Dpn I
Product Size:	100 units
Concentration:	10 U⁄µl
Compatible Buffer:	10X Buffer T
Methylation Sensitivity:	Not dam methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5400 mM KCl0.1 mM EDTA1 mM Dithiothreitol (DTT)0.02% BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.