货号：15240601

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：75 UN

目录价：￥1371.00

市场价格：￥1233.90

会员价格：￥1233.90

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-TGG↓CCA-3´ 3´-ACC↑GGT-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	2 U/ µL
Contents	Bal I 10X Bal I Buffer (1mL) 10X loading dye (1mL)
Reaction Temperature	37°C
Reaction Mixture	Bal I 1 µL 10X Bal I Buffer 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	Enzyme is inactivated by heating at 65°C for 20 minutes.
Effect of DNA Methylation	When the recognition sequence is TGGCCAGG, the enzyme activity is affected by dcm methylase. In this case, general DNA originated from E. coli can not be cleaved by this enzyme.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in 1 hour at 37°C.
10X Reaction Buffer M	10X Bal I Buffer 200 mM Tris-HCl, pH 7.5 70 mM MgCl2 10 mM Dithiothreitol 0.1% BSA
Source	Escherichia coli carrying the plasmid encoding the Bal 1 gene

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)	Bal I Buffer
Relative Activity (%)	20*	20*	20*	20*	40*	100

* The relative activity in Universal Buffers is shown as % of activity in Bal I Buffer.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Product Size:	75 units
Concentration:	2 U⁄µl
Compatible Buffer:	10X Bal I Buffer
Methylation Sensitivity:	dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer:10 mM Tris-HCl, pH 7.5400 mM KCl0.1 mM EDTA1 mM Dithiothreitol (DTT)0.01% BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.